Induction of Nitric Oxide Synthase Activity in Adrenal Cells

The induction of nitric oxide synthase (NOS) II by bacterial lypopolysaccharide (LPS) was studied in a steroidogenic mouse tumor adrenal cell line (Y1). Conditioned media from LPS-stimulated peritoneal macrophages induced an increase in NOS II expression as shown by western and northern blot analysi...

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Bibliographic Details
Published in:Endocrine research 2000-01, Vol.26 (4), p.589-595
Main Authors: Cymeryng, C. B., Lotito, S. P., Colonna, C., Cornejo, M. F., Podestá, E. J.
Format: Article
Language:English
Subjects:
Adrenal Glands - cytology
Adrenal Glands - enzymology
Animals
Cells, Cultured
Culture Media, Conditioned - pharmacology
Lipopolysaccharides - pharmacology
Macrophages, Peritoneal - metabolism
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Nitrites - metabolism
Rats
Steroids - antagonists & inhibitors
Steroids - metabolism
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Summary:The induction of nitric oxide synthase (NOS) II by bacterial lypopolysaccharide (LPS) was studied in a steroidogenic mouse tumor adrenal cell line (Y1). Conditioned media from LPS-stimulated peritoneal macrophages induced an increase in NOS II expression as shown by western and northern blot analysis. Accordingly, in the presence of conditioned media an increase in nitrite levels was observed. In addition, steroid production was significantly decreased. In conclusion, NOS II expression could be induced in steroidogenic cells with a concomitant inhibition of steroid production.
ISSN:0743-5800
1532-4206
DOI:10.3109/07435800009048576