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Metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 and 26,27-hexafluoro-1 alpha,23(S)25-trihydroxyvitamin D3 in ROS17/2.8 cells transfected with a plasmid expressing CYP24
1. To clarify the possibility that the metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 [F6-1,25(OH)2D3] to 26,27-hexafluoro-1 alpha,23(S),25-trihydroxyvitamin D3 [F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to 26,27-hexafluoro-23-oxo-1 alpha,25-dihydroxyvitamin D3 [F6-23-oxo-1,25(O...
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| Published in: | Xenobiotica 2000-11, Vol.30 (11), p.1055-1062 |
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| Main Authors: | , , , , , , , , , , , |
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| container_end_page | 1062 |
| container_issue | 11 |
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| container_title | Xenobiotica |
| container_volume | 30 |
| creator | Miyahara, T Gomyo, S Ueda, Y Ohyama, Y Sigeno, C Kozakai, A Takamura, T Yamazaki, R Higuchi, S Yamamoto, M Sakuma, T Nemoto, N |
| description | 1. To clarify the possibility that the metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 [F6-1,25(OH)2D3] to 26,27-hexafluoro-1 alpha,23(S),25-trihydroxyvitamin D3 [F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to 26,27-hexafluoro-23-oxo-1 alpha,25-dihydroxyvitamin D3 [F6-23-oxo-1,25(OH)2D3] are catalysed by 25-hydroxyvitamin D3 24-hydroxylase (CYP24), ROS17/2.8 cells transfected with a plasmid expressing CYP24 [pSVL-CYP24(+)] and a corresponding blank plasmid [pSLV-CYP24R(-)] were used. 2. Incubation of [1 beta-3H]-F6-1,25(OH)2D3 for 2 and 5 days with ROS17/2.8 cells transfected with pSVL-CYP24(+) generated a metabolite that co-migrated with authentic F6-1,23,25(OH)3D3 in both normal phase and reversed-phase HPLC systems. 3. Incubation of [1 beta-3H]-F6-1,23,25(OH)3D3 for 5 days with pSVL-CYP24(+)- transfected ROS 17/2.8 cells generated a metabolite that co-migrated with authentic F6-23-oxo-1,25(OH)2D3. In contrast, the metabolites F6-1,23,25(OH)3D3 or F6-23-oxo-1,25(OH)2D3 were not generated in the cells transfected with pSVL-CYP24R(-). 4. The results indicate that CYP24 catalyses the conversion of F6-1,25(OH)2D3 to F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to F6-23-oxo-1,25(OH)2D3. |
| doi_str_mv | 10.1080/00498250010002496 |
| format | article |
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To clarify the possibility that the metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 [F6-1,25(OH)2D3] to 26,27-hexafluoro-1 alpha,23(S),25-trihydroxyvitamin D3 [F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to 26,27-hexafluoro-23-oxo-1 alpha,25-dihydroxyvitamin D3 [F6-23-oxo-1,25(OH)2D3] are catalysed by 25-hydroxyvitamin D3 24-hydroxylase (CYP24), ROS17/2.8 cells transfected with a plasmid expressing CYP24 [pSVL-CYP24(+)] and a corresponding blank plasmid [pSLV-CYP24R(-)] were used. 2. Incubation of [1 beta-3H]-F6-1,25(OH)2D3 for 2 and 5 days with ROS17/2.8 cells transfected with pSVL-CYP24(+) generated a metabolite that co-migrated with authentic F6-1,23,25(OH)3D3 in both normal phase and reversed-phase HPLC systems. 3. Incubation of [1 beta-3H]-F6-1,23,25(OH)3D3 for 5 days with pSVL-CYP24(+)- transfected ROS 17/2.8 cells generated a metabolite that co-migrated with authentic F6-23-oxo-1,25(OH)2D3. In contrast, the metabolites F6-1,23,25(OH)3D3 or F6-23-oxo-1,25(OH)2D3 were not generated in the cells transfected with pSVL-CYP24R(-). 4. The results indicate that CYP24 catalyses the conversion of F6-1,25(OH)2D3 to F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to F6-23-oxo-1,25(OH)2D3.</description><identifier>ISSN: 0049-8254</identifier><identifier>DOI: 10.1080/00498250010002496</identifier><identifier>PMID: 11197067</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Calcitriol - analogs & derivatives ; Calcitriol - metabolism ; Cell Line ; Chromatography, High Pressure Liquid ; Cytochrome P-450 Enzyme System - biosynthesis ; Cytochrome P-450 Enzyme System - physiology ; Models, Chemical ; Osteoblasts - metabolism ; Plasmids - metabolism ; Rats ; Steroid Hydroxylases - biosynthesis ; Steroid Hydroxylases - physiology ; Time Factors ; Transfection ; Tumor Cells, Cultured ; Vitamin D3 24-Hydroxylase</subject><ispartof>Xenobiotica, 2000-11, Vol.30 (11), p.1055-1062</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11197067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyahara, T</creatorcontrib><creatorcontrib>Gomyo, S</creatorcontrib><creatorcontrib>Ueda, Y</creatorcontrib><creatorcontrib>Ohyama, Y</creatorcontrib><creatorcontrib>Sigeno, C</creatorcontrib><creatorcontrib>Kozakai, A</creatorcontrib><creatorcontrib>Takamura, T</creatorcontrib><creatorcontrib>Yamazaki, R</creatorcontrib><creatorcontrib>Higuchi, S</creatorcontrib><creatorcontrib>Yamamoto, M</creatorcontrib><creatorcontrib>Sakuma, T</creatorcontrib><creatorcontrib>Nemoto, N</creatorcontrib><title>Metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 and 26,27-hexafluoro-1 alpha,23(S)25-trihydroxyvitamin D3 in ROS17/2.8 cells transfected with a plasmid expressing CYP24</title><title>Xenobiotica</title><addtitle>Xenobiotica</addtitle><description>1. To clarify the possibility that the metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 [F6-1,25(OH)2D3] to 26,27-hexafluoro-1 alpha,23(S),25-trihydroxyvitamin D3 [F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to 26,27-hexafluoro-23-oxo-1 alpha,25-dihydroxyvitamin D3 [F6-23-oxo-1,25(OH)2D3] are catalysed by 25-hydroxyvitamin D3 24-hydroxylase (CYP24), ROS17/2.8 cells transfected with a plasmid expressing CYP24 [pSVL-CYP24(+)] and a corresponding blank plasmid [pSLV-CYP24R(-)] were used. 2. Incubation of [1 beta-3H]-F6-1,25(OH)2D3 for 2 and 5 days with ROS17/2.8 cells transfected with pSVL-CYP24(+) generated a metabolite that co-migrated with authentic F6-1,23,25(OH)3D3 in both normal phase and reversed-phase HPLC systems. 3. Incubation of [1 beta-3H]-F6-1,23,25(OH)3D3 for 5 days with pSVL-CYP24(+)- transfected ROS 17/2.8 cells generated a metabolite that co-migrated with authentic F6-23-oxo-1,25(OH)2D3. In contrast, the metabolites F6-1,23,25(OH)3D3 or F6-23-oxo-1,25(OH)2D3 were not generated in the cells transfected with pSVL-CYP24R(-). 4. The results indicate that CYP24 catalyses the conversion of F6-1,25(OH)2D3 to F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to F6-23-oxo-1,25(OH)2D3.</description><subject>Animals</subject><subject>Calcitriol - analogs & derivatives</subject><subject>Calcitriol - metabolism</subject><subject>Cell Line</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cytochrome P-450 Enzyme System - biosynthesis</subject><subject>Cytochrome P-450 Enzyme System - physiology</subject><subject>Models, Chemical</subject><subject>Osteoblasts - metabolism</subject><subject>Plasmids - metabolism</subject><subject>Rats</subject><subject>Steroid Hydroxylases - biosynthesis</subject><subject>Steroid Hydroxylases - physiology</subject><subject>Time Factors</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>Vitamin D3 24-Hydroxylase</subject><issn>0049-8254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp9kM1OwzAQhH0A0VJ4AC7IJwRS09qOEydHVH4lUBGFA6doE9vUKH_YDrQvxvORinJDXHal2flWo0HoiJIJJQmZEsLThEWEUEII42m8g4YbLehFPkD7zr31h5gytocGlNJUkFgM0de98pA3pXEVbjRm8ZiJYKlWoMuusU1AMZTtEsYsCqRZrqVtVusP46EyNb4IMdTyHyY8XZz1oLd_kP18nC-omLJJggtVlg57C7XTqvBK4k_jlxhwW4KrjMRq1VrlnKlf8ezlgfEDtKuhdOpwu0fo-eryaXYT3M2vb2fnd0FLGfeBoCRNFBQiL1TOtYQklKyACCRXMVEaCuAQEyHjCCKap6nQBWihGdUhSWIVjtDJz9_WNu-dcj6rjNukhVo1nctEXy5LBO-Nx1tjl1dKZq01Fdh19tt0-A0QEX4i</recordid><startdate>200011</startdate><enddate>200011</enddate><creator>Miyahara, T</creator><creator>Gomyo, S</creator><creator>Ueda, Y</creator><creator>Ohyama, Y</creator><creator>Sigeno, C</creator><creator>Kozakai, A</creator><creator>Takamura, T</creator><creator>Yamazaki, R</creator><creator>Higuchi, S</creator><creator>Yamamoto, M</creator><creator>Sakuma, T</creator><creator>Nemoto, N</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200011</creationdate><title>Metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 and 26,27-hexafluoro-1 alpha,23(S)25-trihydroxyvitamin D3 in ROS17/2.8 cells transfected with a plasmid expressing CYP24</title><author>Miyahara, T ; Gomyo, S ; Ueda, Y ; Ohyama, Y ; Sigeno, C ; Kozakai, A ; Takamura, T ; Yamazaki, R ; Higuchi, S ; Yamamoto, M ; Sakuma, T ; Nemoto, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-71098eac7bceb4fda83d2ca5ad4e60efaca4a607d65a51b997fcaf7f21f3086e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Calcitriol - analogs & derivatives</topic><topic>Calcitriol - metabolism</topic><topic>Cell Line</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cytochrome P-450 Enzyme System - biosynthesis</topic><topic>Cytochrome P-450 Enzyme System - physiology</topic><topic>Models, Chemical</topic><topic>Osteoblasts - metabolism</topic><topic>Plasmids - metabolism</topic><topic>Rats</topic><topic>Steroid Hydroxylases - biosynthesis</topic><topic>Steroid Hydroxylases - physiology</topic><topic>Time Factors</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>Vitamin D3 24-Hydroxylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyahara, T</creatorcontrib><creatorcontrib>Gomyo, S</creatorcontrib><creatorcontrib>Ueda, Y</creatorcontrib><creatorcontrib>Ohyama, Y</creatorcontrib><creatorcontrib>Sigeno, C</creatorcontrib><creatorcontrib>Kozakai, A</creatorcontrib><creatorcontrib>Takamura, T</creatorcontrib><creatorcontrib>Yamazaki, R</creatorcontrib><creatorcontrib>Higuchi, S</creatorcontrib><creatorcontrib>Yamamoto, M</creatorcontrib><creatorcontrib>Sakuma, T</creatorcontrib><creatorcontrib>Nemoto, N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Xenobiotica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyahara, T</au><au>Gomyo, S</au><au>Ueda, Y</au><au>Ohyama, Y</au><au>Sigeno, C</au><au>Kozakai, A</au><au>Takamura, T</au><au>Yamazaki, R</au><au>Higuchi, S</au><au>Yamamoto, M</au><au>Sakuma, T</au><au>Nemoto, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 and 26,27-hexafluoro-1 alpha,23(S)25-trihydroxyvitamin D3 in ROS17/2.8 cells transfected with a plasmid expressing CYP24</atitle><jtitle>Xenobiotica</jtitle><addtitle>Xenobiotica</addtitle><date>2000-11</date><risdate>2000</risdate><volume>30</volume><issue>11</issue><spage>1055</spage><epage>1062</epage><pages>1055-1062</pages><issn>0049-8254</issn><abstract>1. To clarify the possibility that the metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 [F6-1,25(OH)2D3] to 26,27-hexafluoro-1 alpha,23(S),25-trihydroxyvitamin D3 [F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to 26,27-hexafluoro-23-oxo-1 alpha,25-dihydroxyvitamin D3 [F6-23-oxo-1,25(OH)2D3] are catalysed by 25-hydroxyvitamin D3 24-hydroxylase (CYP24), ROS17/2.8 cells transfected with a plasmid expressing CYP24 [pSVL-CYP24(+)] and a corresponding blank plasmid [pSLV-CYP24R(-)] were used. 2. Incubation of [1 beta-3H]-F6-1,25(OH)2D3 for 2 and 5 days with ROS17/2.8 cells transfected with pSVL-CYP24(+) generated a metabolite that co-migrated with authentic F6-1,23,25(OH)3D3 in both normal phase and reversed-phase HPLC systems. 3. Incubation of [1 beta-3H]-F6-1,23,25(OH)3D3 for 5 days with pSVL-CYP24(+)- transfected ROS 17/2.8 cells generated a metabolite that co-migrated with authentic F6-23-oxo-1,25(OH)2D3. In contrast, the metabolites F6-1,23,25(OH)3D3 or F6-23-oxo-1,25(OH)2D3 were not generated in the cells transfected with pSVL-CYP24R(-). 4. The results indicate that CYP24 catalyses the conversion of F6-1,25(OH)2D3 to F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to F6-23-oxo-1,25(OH)2D3.</abstract><cop>England</cop><pmid>11197067</pmid><doi>10.1080/00498250010002496</doi><tpages>8</tpages></addata></record> |
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| ispartof | Xenobiotica, 2000-11, Vol.30 (11), p.1055-1062 |
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| language | eng |
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| source | Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Medical Collection (Reading list) |
| subjects | Animals Calcitriol - analogs & derivatives Calcitriol - metabolism Cell Line Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System - biosynthesis Cytochrome P-450 Enzyme System - physiology Models, Chemical Osteoblasts - metabolism Plasmids - metabolism Rats Steroid Hydroxylases - biosynthesis Steroid Hydroxylases - physiology Time Factors Transfection Tumor Cells, Cultured Vitamin D3 24-Hydroxylase |
| title | Metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 and 26,27-hexafluoro-1 alpha,23(S)25-trihydroxyvitamin D3 in ROS17/2.8 cells transfected with a plasmid expressing CYP24 |
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