DNA-dependent adenosinetriphosphatase A is the eukaryotic analog of the bacteriophage T4 gene 44 protein: immunological identity of DNA replication-associated ATPases

We report the construction of three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A. All three of the antibodies react specifically with calf thymus ATPase A and the gene 44 protein from the bacteriophage T4 DNA-dependen...

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Bibliographic Details
Published in:Biochemistry (Easton) 1991-12, Vol.30 (49), p.11490-11494
Main Authors: Mesner, Larry D, Sutherland, William M, Hockensmith, Joel W
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - immunology
Animals
Antibodies, Monoclonal
Biological and medical sciences
DNA Helicases
DNA Replication
DNA, Fungal - biosynthesis
Epitopes - genetics
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Mice
Molecular and cellular biology
Molecular genetics
phage T4
Replication
T-Phages - genetics
Viral Proteins - genetics
Viral Proteins - immunology
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Summary:We report the construction of three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A. All three of the antibodies react specifically with calf thymus ATPase A and the gene 44 protein from the bacteriophage T4 DNA-dependent ATPase. Each of the three anti-ATPase A antibodies appears to recognize a different epitope and none of the antibodies inhibit DNA-dependent ATP hydrolysis by ATPase A. Furthermore, one of the antibodies has been shown to react with two different preparations of HeLa cell DNA-dependent ATPases and a yeast DNA-dependent ATPase, all of which have been implicated in the enzymology of DNA replication. These findings provide strong evidence for the role of ATPase A in DNA replication. These observations lead us to conclude that, apart from the nucleotide binding sites, there are at least three epitopes common to both the bacteriophage and eukaryotic DNA-dependent ATPases that we have examined and that the different preparations of the eukaryotic ATPases contain the same DNA-dependent ATPase.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00113a002