Characterization of four isolates of Bombyx mori nucleopolyhedrovirus

Bombyx mori nucleopolyhedroviruses (BmNPVs), isolated from a sericultural Korean farm, were purified and characterized by their DNA restriction pattern, virus replication, polyhedra production and gene structures. The EcoR I and Sal I fragments showed similar overall patterns with minor difference b...

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Bibliographic Details
Published in:Archives of virology 2000-01, Vol.145 (11), p.2351-2361
Main Authors: Hong, H. K, Woo, S. D, Choi, J. Y, Lee, H. K, Kim, M. H, Je, Y. H, Kang, S. K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acids
Animals
Bacteriology
Biological and medical sciences
Bombyx - virology
Bombyx mori
Bombyx mori NPV
Bombyx mori nucleopolyhedrovirus
Cells, Cultured
Deoxyribonuclease EcoRI - metabolism
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA
Epidemiology
farms
Fundamental and applied biological sciences. Psychology
genes
Korea
Microbiology
Microscopy, Electron, Scanning
Molecular Sequence Data
Mycology
Nuclear polyhedrosis virus
Nucleopolyhedrovirus - classification
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - isolation & purification
Nucleopolyhedrovirus - physiology
Occlusion Body Matrix Proteins
p10 gene
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Polymerase Chain Reaction
Restriction Mapping
Sequence Analysis, DNA
sericulture
Viral Proteins - biosynthesis
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - ultrastructure
Viral Structural Proteins
Virus Replication
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Description
Summary:Bombyx mori nucleopolyhedroviruses (BmNPVs), isolated from a sericultural Korean farm, were purified and characterized by their DNA restriction pattern, virus replication, polyhedra production and gene structures. The EcoR I and Sal I fragments showed similar overall patterns with minor difference but distinguishable patterns in each isolate. There was no significant difference in the virus replication pattern, yield of total polyhedra production and polyhedra morphology, but the yield of released polyhedra by BmNPV-K1 in Bm5 cells was 2 to 5 times higher than that of other isolates. In comparative studies of p10 gene, BmNPV-K1 and K3 had same structure and they encoded a protein consisting of 94 amino acids. Although BmNPV-K2 encoded the same length of amino acids with BmNPV-K1 and K3, it had different structure, and BmNPV-K4 had the p10 gene encoding 70 amino acids.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050070025