Molecular cloning and characterization of prolactin-like protein C complementary deoxyribonucleic acid

In this report, we describe the isolation and characterization of a full length cDNA clone for rat prolactin-like protein C (PLP-C) and describe the expression of PLP-C mRNA in the developing rat placenta. Nucleotide sequence analysis of the PLP-C cDNA clone predicted a mature protein of 238 amino a...

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Bibliographic Details
Published in:The Journal of biological chemistry 1991-12, Vol.266 (34), p.23027-23032
Main Authors: DEB, S, ROBY, K. F, FARIA, T. N, SZPIRER, C, LEVAN, G, KWOK, S. C. M, SOARES, M. J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
cDNA
Chromosome Mapping
Cloning, Molecular
DNA - isolation & purification
Female
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Genetic Complementation Test
homology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequence
Placenta - metabolism
predictions
Pregnancy
Pregnancy Proteins - genetics
prolactin
protein C
Rats
Restriction Mapping
Sequence Alignment
Sequence Homology, Nucleic Acid
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Summary:In this report, we describe the isolation and characterization of a full length cDNA clone for rat prolactin-like protein C (PLP-C) and describe the expression of PLP-C mRNA in the developing rat placenta. Nucleotide sequence analysis of the PLP-C cDNA clone predicted a mature protein of 238 amino acids, including a 30-amino acid signal sequence. The predicted PLP-C amino acid sequence contains seven cysteine residues, three tryptophan residues, and two putative N-linked glycosylation sites. Six of the cysteine residues in PLP-C are located in positions homologous to the cysteines of pituitary prolactin (PRL). Additional sequence similarities with pituitary PRL and other members of the rat placental PRL family are evident. The PLP-C gene was localized to rat chromosome 17. Northern blot analysis showed that the PLP-C cDNA clone specifically hybridized to a 1.0-kilobase mRNA. PLP-C mRNA was first detectable between days 13 and 14 of gestation, peaked by day 18 of gestation, and remained elevated until term. In situ hybridization analysis indicated that PLP-C mRNA was specifically expressed by spongiotrophoblast cells and some trophoblast giant cells in the junctional zone region of rat chorioallantoic placenta.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)54458-6