Selectively conjugated melittins for liposome time-resolved fluoroimmunoassay of theophylline in serum

Theophylline (Th) has been selectively conjugated to the four amino groups of melittin (Mel) by solid phase peptide synthesis. The cytolytic activity of the resultant Th-Mel compounds was tested on liposomes trapping the bovine serum albumin (BSA) conjugate with 4,7-bis(chlorosulfophenyl)-1,10-phena...

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Bibliographic Details
Published in:Fresenius' journal of analytical chemistry 2000-04, Vol.366 (8), p.869-872
Main Authors: IUS, A, BACIGALUPO, M. A, LONGHI, R, MERONI, G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis
Antibodies - immunology
Biological and medical sciences
Calibration
Dose-Response Relationship, Drug
Europium
Fluorescent Dyes - metabolism
Fluoroimmunoassay - methods
General pharmacology
Haptens - immunology
Humans
Liposomes - chemistry
Liposomes - metabolism
Medical sciences
Melitten - analogs & derivatives
Melitten - metabolism
Melitten - pharmacology
Molecular Sequence Data
Permeability - drug effects
Pharmacology. Drug treatments
Phenanthrolines - metabolism
Sensitivity and Specificity
Serum Albumin - metabolism
Substrate Specificity
Theophylline - analogs & derivatives
Theophylline - blood
Theophylline - immunology
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Summary:Theophylline (Th) has been selectively conjugated to the four amino groups of melittin (Mel) by solid phase peptide synthesis. The cytolytic activity of the resultant Th-Mel compounds was tested on liposomes trapping the bovine serum albumin (BSA) conjugate with 4,7-bis(chlorosulfophenyl)-1,10-phenanthrol ine-2,9-dicarboxylic acid (BCPDA). The loss of lytic activity was the highest for Th-K7-Mel. Th-G1-Mel retains almost the same lytic activity as Mel. A homogeneous liposome time-resolved fluoroimmunoassay (LITRFIA) of Th in serum has been carried out with Th-G1-Mel between 5 ng and 10 microg.
ISSN:0937-0633
1432-1130
DOI:10.1007/s002160051587