Deletion of the 19kDa antigen does not alter the protective efficacy of BCG

Expression of the Mycobacterium tuberculosis 19kDa lipoprotein in saprophytic mycobacteria has been found to reduce their ability to prime a protective response to subsequent virulent challenge in the mouse model. The present study was designed to test whether 19kDa expression has an analogous detri...

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Bibliographic Details
Published in:Tubercle and lung disease 2000-01, Vol.80 (6), p.243-247
Main Authors: Yeremeev, V.V., Stewart, G.R., Neyrolles, O., Skrabal, K., Avdienko, V.G., Apt, A.S., Young, D.B.
Format: Article
Language:English
Subjects:
19kDa antigen
Animals
Antigens, Bacterial - genetics
Antigens, Bacterial - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
BCG
BCG Vaccine - genetics
Blotting, Western
Colony Count, Microbial
Electrophoresis, Agar Gel
Electroporation
Female
Gene Deletion
Gene Expression
lipoproteins
Mice
Mice, Inbred C57BL
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Polymerase Chain Reaction
Treatment Outcome
Tuberculosis - prevention & control
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Summary:Expression of the Mycobacterium tuberculosis 19kDa lipoprotein in saprophytic mycobacteria has been found to reduce their ability to prime a protective response to subsequent virulent challenge in the mouse model. The present study was designed to test whether 19kDa expression has an analogous detrimental effect on the efficacy of BCG vaccination. In contrast to the results in saprophytes, neither overexpression of the 19kDa antigen, nor deletion of the endogenous 19kDa gene altered the ability of BCG to protect against M. tuberculosis challenge in a mouse model.
ISSN:0962-8479
1532-219X
DOI:10.1054/tuld.2000.0252