Cryopreservation of Rhesus Macaque (Macaca mulatta) Spermatozoa and Their Functional Assessment by in Vitro Fertilization

Although spermatozoa from several species of nonhuman primates have been cryopreserved, there has been no report of success with rhesus macaque spermatozoa as judged by functional assays. Two Tris–egg yolk freezing media, TEST and TTE, which have been successfully used for cynomolgus macaque (Macaca...

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Published in:Cryobiology 2000-11, Vol.41 (3), p.232-240
Main Authors: Si, Wei, Zheng, Ping, Tang, Xianghui, He, Xiechao, Wang, Hong, Bavister, Barry D, Ji, Weizhi
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
capacitation
Cryopreservation
cryoprotectants
embryo development
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
glycerol
Gynecology. Andrology. Obstetrics
in vitro fertilization
Macaca fascicularis
Macaca mulatta
Male
Mammalian male genital system
Medical sciences
Morphology. Physiology
plasma membrane
semen
Sperm Motility
spermatozoa
Spermatozoa - physiology
Sterility. Assisted procreation
sugar
Vertebrates: reproduction
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Summary:Although spermatozoa from several species of nonhuman primates have been cryopreserved, there has been no report of success with rhesus macaque spermatozoa as judged by functional assays. Two Tris–egg yolk freezing media, TEST and TTE, which have been successfully used for cynomolgus macaque (Macaca fascicularis) spermatozoa, were compared for cryopreservation of spermatozoa from four rhesus macaques (Macaca mulatta). The postthaw motility (percentage and duration) of spermatozoa cryopreserved in TTE was much higher than that for spermatozoa cryopreserved in TEST. The function of sperm cryopreserved in TTE was evaluated by in vitro fertilization of oocytes collected from gonadotropin-stimulated prepubertal rhesus macaques. Of the inseminated oocytes, 82 ± 13% were fertilized and 63 ± 22 and 39 ± 21% of the resulting zygotes developed into morulae and blastocysts, respectively. These results indicate that rhesus macaque spermatozoa can be effectively cryopreserved in TTE medium. This finding will facilitate the application of in vivo and in vitro assisted reproductive technologies in this species.
ISSN:0011-2240
1090-2392
DOI:10.1006/cryo.2000.2283