Modified screen-printed electrodes for the investigation of the interaction of non-electroactive quinazoline derivatives with DNA

Five morpholino-quinazoline derivatives have been investigated voltammetrically using a competition with the tris(o-phenanthroline) cobalt(III) redox marker for the accumulation at dsDNA modified screen-printed electrodes. An association of quinazolines with DNA was observed at the modified electrod...

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Bibliographic Details
Published in:Fresenius' journal of analytical chemistry 2000-06, Vol.367 (4), p.364-368
Main Authors: LABUDA, J, BUCKOVA, M, JANTOVA, S, STEPANEK, I, SURUGIU, I, DANIELSSON, B, MASCINI, M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Calibration
DNA - chemistry
Electrochemistry
Electrodes
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Molecular Structure
Quinazolines - chemistry
Various methods and equipments
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Summary:Five morpholino-quinazoline derivatives have been investigated voltammetrically using a competition with the tris(o-phenanthroline) cobalt(III) redox marker for the accumulation at dsDNA modified screen-printed electrodes. An association of quinazolines with DNA was observed at the modified electrodes polarized by the negative potential of -0.4 V vs. Ag/AgCl. This was confirmed by a potentiometric stripping analysis based on the DNA guanine signal. Calibration curves for quinazolines within a concentration range of micromol/L were obtained with DP voltammetry using 5 x 10(-7) mol/L Co(phen)3(3+) marker. The quinazolines exhibit no effect on the DNA complex with the fluorescent thiazole orange derivative TO-PRO-3. The role of the accumulation potential in the association interaction with DNA is discussed.
ISSN:0937-0633
1432-1130
DOI:10.1007/s002160000388