Analysis of HLA-DR and -DQ gene polymorphisms in Sudanese patients with type 1 (insulin-dependent) diabetes

In this study we report for the first time, the molecular analysis of HLA-DR and -DQ gene frequencies in a large cohort of well characterized type 1 (insulin-dependent) diabetes mellitus (IDDM) patients (n = 72), and ethnically matched controls (n = 59) collected in sub-Saharan Africa. High molecula...

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Bibliographic Details
Published in:Immunogenetics (New York) 1991, Vol.34 (6), p.366-371
Main Authors: MAGZOUB, M. M. A, STEPHENS, H. A. F, GALE, E. A. M, BOTTAZZO, G. F
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Blotting, Southern
Child
Diabetes Mellitus, Type 1 - genetics
Diabetes Mellitus, Type 1 - immunology
Diabetes. Impaired glucose tolerance
DNA Probes
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Female
Gene Frequency
Haplotypes
HLA-DQ Antigens - genetics
HLA-DR Antigens - genetics
Humans
Immunophenotyping
Male
Medical sciences
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Sudan
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Summary:In this study we report for the first time, the molecular analysis of HLA-DR and -DQ gene frequencies in a large cohort of well characterized type 1 (insulin-dependent) diabetes mellitus (IDDM) patients (n = 72), and ethnically matched controls (n = 59) collected in sub-Saharan Africa. High molecular mass DNA was prepared and analyzed in Southern blots with DRB1, DQA1, and DQB1 probes. By identifying DR and DQ allele-specific restriction fragment length polymorphisms (RFLPs), we have shown a strong positive association between IDDM and the Asp 57- DQB1 allele *0201 (DQw2). A rare DR4, DQw2 haplotype was also identified at high frequency in the IDDM cohort. We can now confirm that the association between Asp 57-DQB1 alleles and IDDM, previously reported in ethnically diverse cohorts collected in Western Europe, North America, and South Asia, is also present in an IDDM cohort collected in Africa.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF01787486