Gcn4 Activator Targets Gcn5 Histone Acetyltransferase to Specific Promoters Independently of Transcription

Histone acetylation correlates well with transcriptional activity, and histone acetyltransferases (HATs) selectively regulate subsets of target genes by mechanisms that remain unclear. Here, we provide in vivo evidence that the yeast transcriptional activator Gcn4 recruits Gcn5 HAT complexes to sele...

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Bibliographic Details
Published in:Molecular cell 2000-12, Vol.6 (6), p.1309-1320
Main Authors: Kuo, Min-Hao, vom Baur, Elmar, Struhl, Kevin, Allis, C.David
Format: Article
Language:English
Subjects:
Acetylation
Acetyltransferases - metabolism
Chromatin - genetics
Chromatin - metabolism
DNA-Binding Proteins - metabolism
Fungal Proteins - chemistry
Fungal Proteins - metabolism
Gcn4 protein
Gcn5 protein
Gene Expression Regulation, Fungal
Genes, Fungal - genetics
Genome, Fungal
Histone Acetyltransferases
Histones - metabolism
Precipitin Tests
Promoter Regions, Genetic - genetics
Protein Kinases - chemistry
Protein Kinases - metabolism
Protein Structure, Tertiary
Response Elements - genetics
RNA, Fungal - analysis
RNA, Fungal - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Substrate Specificity
Trans-Activators - metabolism
Transcription, Genetic - genetics
Transcriptional Activation
Yeasts - enzymology
Yeasts - genetics
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Summary:Histone acetylation correlates well with transcriptional activity, and histone acetyltransferases (HATs) selectively regulate subsets of target genes by mechanisms that remain unclear. Here, we provide in vivo evidence that the yeast transcriptional activator Gcn4 recruits Gcn5 HAT complexes to selective promoters positioned in natural or ectopic locations, thereby creating local domains of histone H3 hyperacetylation and subsequent transcriptional activation. A significant portion of the Gcn4-targeted histone acetylation by Gcn5 is independent of transcriptional activity. These observations provide strong evidence for promoter-selective, targeted histone acetylation by Gcn5 that facilitates transcription in a causal fashion. In addition, Gcn5 also functions in an untargeted manner to acetylate H3 on a genome-wide scale.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(00)00129-5