Experimental setup for the determination of analytes contained in ultrasonically levitated drops

An automated technique for the determination of analytes in an ultrasonically levitated sample of 2 to 5 microL volume has been developed. Contactless dosing of reagents or solvents into an ultrasonically levitated drop was realized via piezoelectric micropumps. Drop size was continually controlled...

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Bibliographic Details
Published in:Fresenius' journal of analytical chemistry 2000-09, Vol.368 (2-3), p.125-129
Main Authors: ROHLING, O, WEITKAMP, C, NEIDHART, B
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Exact sciences and technology
General, instrumentation
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Summary:An automated technique for the determination of analytes in an ultrasonically levitated sample of 2 to 5 microL volume has been developed. Contactless dosing of reagents or solvents into an ultrasonically levitated drop was realized via piezoelectric micropumps. Drop size was continually controlled with a programmed CCD camera. A diode array spectrometer designed for the use with optical fibers was used for absorption and fluorescence measurements. Determinations via direct absorption measurements following the method of standard addition and acid-base titrations with an absorption indicator and a fluorescent indicator were carried out. The mean consumption of sodium hydroxide added via a piezoelectric micropump in five successive titrations with 18 nmol of sulfuric acid inside a levitated drop (indicator bromothymol blue) had a relative standard deviation of 0.7% and differed only by 0.2% from the expected value.
ISSN:0937-0633
1432-1130
DOI:10.1007/s002160000391