Differentiation of Streptococcus uberis from Streptococcus parauberis by polymerase chain reaction and restriction fragment length polymorphism analysis of 16S ribosomal DNA

Streptococcus uberis type II has been proposed recently as a separate species designated Streptococcus parauberis (A. M. Williams and M. D. Collins, J. Appl. Bacteriol. 68:485-490, 1990). Differentiation of S. parauberis from S. uberis has been possible only by DNA-DNA hybridization or 16S rRNA sequ...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1991-12, Vol.29 (12), p.2774-2778
Main Authors: Jayarao, B.M. (University of Tennessee, Knoxville, TN), Dore, J.J.E. Jr, Baumbach, G.A, Matthews, K.R, Oliver, S.P
Format: Article
Language:English
Subjects:
ADN
Animals
Bacteriology
Base Sequence
BIOCHIMIE
Biological and medical sciences
BIOQUIMICA
Cattle
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
Female
Fundamental and applied biological sciences. Psychology
GLANDE MAMMAIRE
GLANDULAS MAMARIAS
MAMMITE
MASTITIS
Microbiology
Molecular Sequence Data
POLIMORFISMO
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
POLYMORPHISME
restriction fragment length polymorphism
RIBOSOMAS
RIBOSOME
RNA, Ribosomal, 16S - genetics
rRNA 16S
Species Specificity
STREPTOCOCCUS
Streptococcus - classification
Streptococcus - genetics
Streptococcus - metabolism
Streptococcus parauberis
STREPTOCOCCUS UBERIS
Systematics
VACAS LECHERAS
VACHE LAITIERE
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Summary:Streptococcus uberis type II has been proposed recently as a separate species designated Streptococcus parauberis (A. M. Williams and M. D. Collins, J. Appl. Bacteriol. 68:485-490, 1990). Differentiation of S. parauberis from S. uberis has been possible only by DNA-DNA hybridization or 16S rRNA sequencing, since the biochemical and serological characteristics of the two species are indistinguishable. A simple and reliable technique was developed for differentiating S. parauberis (S. uberis type II [ATCC 13386]) from S. uberis (S. uberis type I [ATCC 9927, ATCC 13387, and ATCC 27958]) by restriction fragment length polymorphism (RFLP) analysis of 1.4-kb 16S ribosomal DNA (rDNA). Oligonucleotide primers complementary to 16S rRNA genes were used to amplify 16S ribosomal gene fragments from genomic DNA by polymerase chain reaction. The 1.4-kb 16S rDNA fragment was digested with ScaI, NspI, DdeI, and AvaII restriction endonucleases. Restriction fragments produced by all four restriction endonucleases were characteristic for each species. RFLP analysis of 16S rDNA from 24 "S. uberis" isolates obtained from mammary secretions of dairy cows indicated that all 24 isolates were indeed S. uberis
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.29.12.2774-2778.1991