Inositol and inositol 1,4,5-trisphosphate content of Down syndrome fibroblasts exhibiting enhanced inositol uptake

Fibroblasts from individuals with Down syndrome (DS; trisomy 21) exhibit increased inositol uptake. Here we examine the relationship between this increase in uptake and mass levels of free inositol and inositol 1,4,5-trisphosphate (IP 3) in DS fibroblasts. We report that human fibroblasts contain hi...

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Bibliographic Details
Published in:FEBS letters 1991-12, Vol.295 (1), p.43-47
Main Authors: Fruen, Bradley R., Lester, Bruce R.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Transport
Cell physiology
Cells, Cultured
Down syndrome
Down Syndrome - metabolism
Down's syndrome
fibroblasts
Fibroblasts - metabolism
Fundamental and applied biological sciences. Psychology
Human fibroblast
Humans
Inositol
Inositol - metabolism
Inositol 1,4,5-trisphosphate
Inositol 1,4,5-Trisphosphate - metabolism
IP3
Kinetics
man
Membrane and intracellular transports
Molecular and cellular biology
Reference Values
Skin - metabolism
Transport
uptake
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Summary:Fibroblasts from individuals with Down syndrome (DS; trisomy 21) exhibit increased inositol uptake. Here we examine the relationship between this increase in uptake and mass levels of free inositol and inositol 1,4,5-trisphosphate (IP 3) in DS fibroblasts. We report that human fibroblasts contain high levels of free inositol which are not significantly affected by the increase in inositol uptake associated with DS. In addition, increased uptake is accompanied by increased efflux of radiolabelled inositol from DS cells. Neither basal nor bradykinin-stimulated IP 3 levels in DS cells differ significantly from normal values. This work highlights the usefulness of the DS cell in uncovering the role of transport across the plasma membrane in cellular inositol homeostasis.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)81380-Q