Cloning and functional characterization of a cocaine-sensitive dopamine transporter

We report the cloning of a rat cDNA encoding a functional dopamine transporter. This cDNA, derived from an intron-containing gene, encodes a protein of 620 amino acids. Hydropathicity analysis of the protein sequence suggests the presence of 12 putative transmembrane domains. The protein displays co...

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Bibliographic Details
Published in:FEBS letters 1991-12, Vol.295 (1), p.149-154
Main Authors: Giros, Bruno, El Mestikawy, Salah, Bertrand, Lucie, Caron, Marc G.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Base Sequence
Binding and carrier proteins
Biological and medical sciences
Blotting, Southern
Brain - physiology
Carrier Proteins - drug effects
Carrier Proteins - genetics
Carrier Proteins - metabolism
cDNA
Cell Line
Cloning, Molecular - methods
Cocaine - pharmacology
DNA - genetics
DNA - isolation & purification
Dopamine - metabolism
Dopamine Plasma Membrane Transport Proteins
Dopamine transporter
expression
function
Fundamental and applied biological sciences. Psychology
Gene Library
genes
Kinetics
Membrane Glycoproteins
Membrane Proteins - genetics
Membrane Transport Proteins
Molecular Sequence Data
Molecular Weight
Nerve Tissue Proteins
Oligodeoxyribonucleotides
Polymerase Chain Reaction - methods
Proteins
Rats
Recombinant Proteins - drug effects
Recombinant Proteins - metabolism
Restriction Mapping
RNA, Messenger - analysis
RNA, Messenger - genetics
Substantia nigra pars compacta
Transfection
Transmembrane domain
Ventral tegmental
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Description
Summary:We report the cloning of a rat cDNA encoding a functional dopamine transporter. This cDNA, derived from an intron-containing gene, encodes a protein of 620 amino acids. Hydropathicity analysis of the protein sequence suggests the presence of 12 putative transmembrane domains. The protein displays considerable identity with transporters for noradrenaline and GABA (64 and 30%, respectively). Transient expression of the cDNA in COS7 cells directs the expression of dopamine uptake activity with appropriate pharmacology and in a sodium-dependent fashion. In situ hybridization reveals that the mRNA for this transporter is expressed in the substantia nigra and ventral tegmental area, regions that contain dopaminergic cell bodies.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)81406-X