Molecular and Cell Biology of Skin Wound Healing in a Pig Model

To define the pattern of change at the molecular and cellular levels during the healing of exci-sional skin wounds in the skeletally immature pig, mRNA levels for relevant molecules were assessed by semiquantitative RT-PCR using porcine specific primer sets and RNA isolated from normal skin and samp...

Full description

Saved in:
Bibliographic Details
Published in:Connective tissue research 2000, Vol.41 (3), p.195-211
Main Authors: Wang, Jian Fei, Olson, Merle E., Reno, Carolyn R., Kulyk, William, Wright, J. Barry, Hart, David A.
Format: Article
Language:English
Subjects:
Animals
cellular biology
Collagen - genetics
Disease Models, Animal
DNA - metabolism
Female
Gene Expression
Heat-Shock Proteins - genetics
HSP47 Heat-Shock Proteins
Humans
Interleukin-1 - genetics
Metalloendopeptidases - genetics
mRNA
Pig
Proteoglycans - genetics
RNA - metabolism
RNA, Messenger - metabolism
skin
Skin - cytology
Skin - immunology
Skin - injuries
Skin - metabolism
Swine
Tissue Inhibitor of Metalloproteinase-1 - genetics
Transforming Growth Factor beta - genetics
wound healing
Wound Healing - immunology
Wound Healing - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To define the pattern of change at the molecular and cellular levels during the healing of exci-sional skin wounds in the skeletally immature pig, mRNA levels for relevant molecules were assessed by semiquantitative RT-PCR using porcine specific primer sets and RNA isolated from normal skin and samples at various time post-wounding. Analysis of cellular change was assessed by DNA quantification and histology of tissue sections. The results demonstrated that the changes in the pattern of RNA and DNA content of the scar tissue were consistent with the observed increasing cellularity. The mRNA levels for collagen I, III, HSP47, IL-1, TGF-P, MMP-1, -2 and -9, TIMP-1, -2, and-4, PAI-1, versican were significantly elevated during healing; levels for biglycan and fibromodulin were not significantly altered; and the mRNA levels for TIMP-3 were depressed. These findings suggest that skin wound healing is a series of complex matrix-cell interactions that involve cellular migration and inflammation, followed by proliferation of fibroblasts with new collagen synthesis, and lastly tissue remodeling of the scar.
ISSN:0300-8207
1607-8438
DOI:10.3109/03008200009005290