Regional genetic variation in the major sperm protein genes of Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea)

Onchocerca volvulus and Mansonella ozzardi are two human filarial parasites present in South and Central America. In the Brazilian Amazonia they are found in sympatry, and the lack of clear morphological diagnostic characters in the microfilariae hinders their identification. The major sperm protein...

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Bibliographic Details
Published in:International journal for parasitology 2000-12, Vol.30 (14), p.1459-1465
Main Authors: Morales Hojas, Ramiro, Post, Rory J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Biological evolution
Brazil
Filarial parasites
Fundamental and applied biological sciences. Psychology
Genetic Variation
Genetics of eukaryotes. Biological and molecular evolution
Helminth Proteins - genetics
Humans
Invertebrates
major sperm protein
Major sperm protein evolution
Major sperm protein genes
Male
Mansonella - genetics
Mansonella ozzardi
Molecular Sequence Data
MSP gene
Nemathelminthia. Plathelmintha
Onchocerca volvulus
Onchocerca volvulus - genetics
Onchocerciasis
Sequence Alignment
Species identification
Systematics. Geographical distribution
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Summary:Onchocerca volvulus and Mansonella ozzardi are two human filarial parasites present in South and Central America. In the Brazilian Amazonia they are found in sympatry, and the lack of clear morphological diagnostic characters in the microfilariae hinders their identification. The major sperm protein (MSP) gene of both species has been sequenced and characterised to determine its potential as a molecular diagnostic character. The length of the MSP gene is different in each species, and this could be used to detect and differentiate them by running the polymerase chain reaction (PCR) product in an agarose gel. Two major gene groups were identified in O. volvulus with a genetic distance of 6% between them. In M. ozzardi only one major group of genes was observed. The high similarity between the protein amino acid sequence of both filarial species confirms that the MSP has been highly conserved through nematode evolution.
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(00)00117-X