Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp

• Published in: Biosensors & bioelectronics 2000-06, Vol.15 (3), p.167-172
• Main Authors: Park, In-Seon, Kim, Woo-Yeon, Kim, Namsoo
• Format: Article
• Language: English
• Subjects: Animals; Anti- Salmonella antibody immobilization; Antibodies, Bacterial; Biological and medical sciences; Biosensing Techniques - methods; Biosensing Techniques - statistics & numerical data; Biosensors; Biotechnology; Electrochemistry; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Milk - microbiology; Model sample measurement; Quartz; Quartz crystal microbalance system; Salmonella - immunology; Salmonella - isolation & purification; Salmonella detection; Salmonella typhimurium; Salmonella typhimurium - immunology; Salmonella typhimurium - isolation & purification; Sensitivity and Specificity; Sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate thiolation; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(00)00053-1

A quartz crystal microbalance (QCM) system detecting Salmonella spp. was developed by an anti- Salmonella antibody immobilization onto one gold surface of a piezoelectric quartz crystal surface with sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) thiolation. The optimum temperature and pH for the antibody-immobilized sensor were 35°C and 7.2, respectively. The frequency shifts obtained were correlated with the Salmonella concentrations in the range 3.2×10 6–4.8×10 8 CFU per ml. The system was quite specific to Salmonella spp. and applicable for repetitive use after a regeneration step employing 1.2 M NaOH. A model sample measurement was done for a market milk spiked with Salmonella typhimurium.