Mutant T cell lines as model systems for the dissection of T cell antigen receptor signaling pathways

T cell antigen receptor (TCR) ligation triggers a cascade of intracellular signaling events that culminate in T cell activation, cytokine gene expression, differentiation, or apoptosis. Many of the enzymes and adapter proteins responsible for signal propagation from the cell surface TCR to the cytop...

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Bibliographic Details
Published in:Immunologic research 2000-01, Vol.22 (2-3), p.95-117
Main Author: Abraham, R T
Format: Article
Language:English
Subjects:
Adapter proteins
Adaptor proteins
Animals
Antigens
Apoptosis
Cell activation
Cell differentiation
Cell lines
Cell surface
Cytokines
Cytoplasm
Gene expression
Humans
Immunology
Isoenzymes - deficiency
Isoenzymes - genetics
Isoenzymes - physiology
Jurkat Cells
Kinases
Lymphocyte Activation
Lymphocytes
Lymphocytes T
Mutants
Mutation
Phospholipase C
Phospholipase C gamma
Protein-tyrosine kinase
Protein-Tyrosine Kinases - deficiency
Protein-Tyrosine Kinases - genetics
Protein-Tyrosine Kinases - physiology
Proteins
Receptors
Receptors, Antigen, T-Cell - immunology
Signal Transduction
T cell receptors
T-Lymphocytes - immunology
Type C Phospholipases - deficiency
Type C Phospholipases - genetics
Type C Phospholipases - physiology
Tyrosine
ZAP-70 protein
ZAP-70 Protein-Tyrosine Kinase
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Description
Summary:T cell antigen receptor (TCR) ligation triggers a cascade of intracellular signaling events that culminate in T cell activation, cytokine gene expression, differentiation, or apoptosis. Many of the enzymes and adapter proteins responsible for signal propagation from the cell surface TCR to the cytoplasm and nucleus have now been identified and molecularly cloned. However, a comprehensive understanding of the regulation and functions of these signaling proteins in T cells remains a major challenge. Our laboratory has approached this problem through the generation of a panel of Jurkat T cell-derived somatic mutants that fail to express several critical elements in the TCR-linked signaling cascade. This review highlights the use of mutant T cell lines for functional characterizations of two of these signaling proteins--the ZAP-70 tyrosine kinase and phospholipase C-gamma1.
ISSN:0257-277X
0257-277X
1559-0755
DOI:10.1385/IR:22:2-3:95