Structure of the O-antigen of Francisella tularensis strain 15

• Published in: Carbohydrate research 1991-07, Vol.214 (2), p.289-297
• Main Authors: Vinogradov, Eugeny V., Shashkov, Alexander S., Knirel, Yuriy A., Kochetkov, Nikolay K., Tochtamysheva, Natalya V., Averin, Sergey F., Goncharova, Olga V., Khlebnikov, Valentin S.
• Format: Article
• Language: English
• Subjects: Analysis of complex biological substances; Analytical, structural and metabolic biochemistry; Animal; Antigens, Bacterial - chemistry; Biological and medical sciences; Carbohydrate Conformation; Carbohydrate Sequence; Francisella tularensis - chemistry; Francisella tularensis - immunology; Fundamental and applied biological sciences. Psychology; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Polysaccharides, Bacterial - chemistry; Polysaccharides, Bacterial - immunology; Pseudomonas aeruginosa - chemistry; Pseudomonas aeruginosa - immunology; Shigella dysenteriae - chemistry; Shigella dysenteriae - immunology; Species Specificity
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/0008-6215(91)80036-M

The O-specific polysaccharide, obtained by mild acid degradation of the lipopolysaccharide of Francisella tularensis strain 15, contained 2-acetamido-2,6-dideoxy- d-glucose ( d-QuiNAc), 4,6-dideoxy-4-formamido- d-glucose d-Qui4NFm), and 2-acetamido-2-deoxy- d-galacturonamide ( d-GalNAcAN) in the ratios 1:1:2. Tri- and tetra-saccharide fragments were obtained on treatment of the polysaccharide with anhydrous hydrogen fluoride and partial hydrolysis with 0.1 m hydrochloric acid, respectively. On the basis of 1H- and 13C-n.m.r. spectroscopy of the polysaccharide and the saccharides, it was concluded that the O-antigen had the structure: →4)-α- d-Gal pNAcAN-(1→4)-α- d-Gal pNAcAN-(1→3)-β- d-Qui pNAc-(1→2)-β- d-Qui p4NFm-(1→. This O-antigen is related in structure to those of Pseudomonas aeruginosa O6, immunotype 1, and IID 1008, and Shigella dysenteriae type 7.