Surfactant inhibition of bacterial growth on solid anthracene

• Published in: Biodegradation (Dordrecht) 2000-01, Vol.11 (5), p.341-347
• Main Authors: Chen, P, Pickard, M A, Gray, M R
• Format: Article
• Language: English
• Subjects: Anthracene; Anthracenes - metabolism; Bioremediation; Carbon sources; Cell Division - drug effects; Dose-Response Relationship, Drug; Glucose - metabolism; Growth rate; Micelles; Microbiology; Mycobacterium; Mycobacterium - drug effects; Mycobacterium - growth & development; Mycobacterium - metabolism; Octoxynol - metabolism; Octoxynol - pharmacology; Pseudomonas; Pseudomonas - drug effects; Pseudomonas - growth & development; Pseudomonas - metabolism; Soil contamination; Soil Microbiology; Surface-Active Agents - metabolism; Surface-Active Agents - pharmacology; Surfactants
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1023/A:1011160004678

Surfactants have been proposed as a promising method to enhance bioremediation of hydrophobic compounds in contaminated soils. However, the results of effects of surfactants on bioremediation are not consistent. This study showed that Triton X-100 at low concentration (0.024 mM or 0.09 CMC) inhibited the rate of growth of either a Mycobacterium sp. or a Pseudomonas sp. on solid anthracene as sole carbon source. Recovery of microbial growth rate could be achieved by dilution of surfactants, while addition of more surfactant gave an immediate decrease in growth rate. No inhibition of growth by Triton X-100 was observed with growth on glucose. The surfactant sorbed onto the surfaces of both the cells and the anthracene particles, which could inhibit uptake of anthracene. The results were consistent with the hypothesis that inhibition of microbial adhesion of cells to anthracene was responsible for the inhibition of growth by Triton X-100.