Platelet Aggregation by Strains of Enterococci

The platelet aggregation capability of whole cells of Enterococcus faecalis, E. faecium and E. avium was tested. The optimum ratios of bacteria to platelets in E. faecalis (strain SMU-37), E. faecium (strain SMU-138) and E. avium (strain SMU-197) were 1.0, 1.2 and 2.0, respectively. During the plate...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1991, Vol.35(11), pp.933-942
Main Authors: Usui, Yukio, Ichiman, Yoshitoshi, Suganuma, Masaru, Yoshida, Kosaku
Format: Article
Language:English
Subjects:
Animals
Arachidonic Acid - metabolism
Bacteriology
Biological and medical sciences
Enterococcus - physiology
Enterococcus avium
Enterococcus faecalis
Enterococcus faecium
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Plasma - physiology
Platelet Aggregation - physiology
Rabbits
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Summary:The platelet aggregation capability of whole cells of Enterococcus faecalis, E. faecium and E. avium was tested. The optimum ratios of bacteria to platelets in E. faecalis (strain SMU-37), E. faecium (strain SMU-138) and E. avium (strain SMU-197) were 1.0, 1.2 and 2.0, respectively. During the platelet aggregation induced by the three strains of enterococci, 65-69% of total serotonin was released. The aggregation was totally inhibited by ethylenediaminetetraacetate (10mM) and apyrase (1mg/ml), while no effect was shown by aspirin (10mM), indomethacin (10mM) and quinacrine (1mM). By pretreatment of platelet-poor plasma with heat (56C, 30min) or zymosan, the reactivities with platelets of each strain of species were markedly diminished. These results suggest that enterococci-induced platelet aggregation was an ion-dependent, cyclooxygenase-insensitive event, and plasma component(s) was (were) required for the reaction.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1991.tb01615.x