Production and Partial Characterization of Monoclonal Antibodies Specific for the Gamonts of Eimeria tenella

Thirteen hybridomas secreting monoclonal antibodies (Mabs) specific for the sexual stages (gamonts) of Eimeria tenella were produced by fusing spleen cells of gamont-immunized RBF/Dn mice with FOX-NY myeloma cells. A Mab subisotype profile revealed 1 IgG2a and 12 IgG1. All Mabs demonstrated a simila...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of parasitology 1991-12, Vol.77 (6), p.1012-1015
Main Authors: Larsen, Nancy C., Rasmussen, Kathleen R., Healey, Mark C.
Format: Article
Language:English
Subjects:
Animal protozoal diseases
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - immunology
Antibodies, Protozoan - immunology
Antibody Specificity
Antigens
Biological and medical sciences
Cecum - parasitology
Chickens - parasitology
Coccidiosis
Coccidiosis - prevention & control
Coccidiosis - veterinary
Eimeria tenella
Eimeria tenella - immunology
Fluorescent Antibody Technique
Gametocytes
Hybridomas
Immunization, Passive
Infections
Infectious diseases
Intestinal Diseases, Parasitic - prevention & control
Intestinal Diseases, Parasitic - veterinary
Lesions
Medical sciences
Monoclonal antibodies
Oocysts
Parasites
Parasitic diseases
Parasitism
Parasitology
Poultry Diseases - prevention & control
Protozoal diseases
Research Notes
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thirteen hybridomas secreting monoclonal antibodies (Mabs) specific for the sexual stages (gamonts) of Eimeria tenella were produced by fusing spleen cells of gamont-immunized RBF/Dn mice with FOX-NY myeloma cells. A Mab subisotype profile revealed 1 IgG2a and 12 IgG1. All Mabs demonstrated a similar binding pattern when incubated with parasitic gamonts as determined by the indirect fluorescent antibody test. Ascitic fluid containing Mab GD9 (IgG1) was produced and used to immunize chicks passively per os. There was a 34% decrease (P < 0.05) in oocyst output from immunized chicks when compared to control chicks. Passively immunized chicks also had reduced cecal lesion scores when compared to control chicks. These results suggest that Mab GD9 partially inhibited the fertilization process of E. tenella.
ISSN:0022-3395
1937-2345
DOI:10.2307/3282759