BanAI a new isoschizomer of the type II restriction endonuclease HaeIII discovered in a Bacillus anthracis isolate from Amazon Basin

Bacillus anthracis was isolated and identified from a bacterial collection of samples from the Amazon river bank. Type II restriction endonuclease activity was detected in this prokaryote, the enzyme was purified, the molecular mass of the native protein estimated by gel filtration, and optima pH, t...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-09, Vol.215 (1), p.97-101
Main Authors: Chies, Jocelei M, de O. Dias, Ana C, Maia, Helio M.M, Astolfi-Filho, Spartaco
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Bacillus anthracis
Bacillus anthracis - enzymology
Bacillus anthracis - genetics
Bacterial Proteins - genetics
Bacterial Proteins - isolation & purification
BanAI
basins
Biological and medical sciences
Biotechnology
Brazil
Deoxyribonucleases, Type II Site-Specific - genetics
Deoxyribonucleases, Type II Site-Specific - isolation & purification
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
gel chromatography
Genetic engineering
Genetic technics
HaeIII
Hydrolases
Isoschizomers
Methods. Procedures. Technologies
Miscellaneous
molecular weight
prokaryotic cells
quality control
restriction endonucleases
sequence analysis
Synthetic digonucleotides and genes. Sequencing
temperature
Type II restriction endonuclease
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Summary:Bacillus anthracis was isolated and identified from a bacterial collection of samples from the Amazon river bank. Type II restriction endonuclease activity was detected in this prokaryote, the enzyme was purified, the molecular mass of the native protein estimated by gel filtration, and optima pH, temperature and salt requirements were determined. Quality control assays showed complete absence of ‘non-specific nucleases’. Restriction cleavage analysis and DNA sequencing of restriction fragments allowed unequivocal demonstration of 5′-GG↓CC-3′ as the recognition sequence. This enzyme was named BanAI and is therefore an isoschizomer of the prototype restriction endonuclease HaeIII. This is the first report of a type II restriction endonuclease identified, purified from a natural isolate of B. anthracis.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(02)00917-5