DNA methylation, ageing and ribosomal genes activity

DNA methylation is an epigenetic mechanism controlling of gene expression. We studied the effect of a demethylation agent 5-azacytidine, on ribosomal gene activity observed by Nucleolus Staining Giemsa in the chromosomes of eight Alzheimer's disease patients, eight elderly healthy individuals a...

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Bibliographic Details
Published in:Biogerontology (Dordrecht) 2000-01, Vol.1 (4), p.357-361
Main Authors: de Carvalho, C V, Payão, S L, Smith, M A
Format: Article
Language:English
Subjects:
Adult
Aged
Aging
Aging - genetics
Alzheimer Disease - genetics
Alzheimer's disease
Azacitidine - pharmacology
Azacytidine
Cell culture
Cells, Cultured
Chromosomes
Chromosomes, Human, Pair 22
Demethylation
Deoxyribonucleic acid
DNA
DNA Methylation
DNA, Ribosomal - metabolism
epigenetics
Geriatrics
Humans
Lymphocytes
Metaphase
Neurodegenerative diseases
Nucleoli
Ribosomes - metabolism
Transcription
Transcription, Genetic - drug effects
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Summary:DNA methylation is an epigenetic mechanism controlling of gene expression. We studied the effect of a demethylation agent 5-azacytidine, on ribosomal gene activity observed by Nucleolus Staining Giemsa in the chromosomes of eight Alzheimer's disease patients, eight elderly healthy individuals and eight young healthy controls. Metaphase cells were obtained from lymphocyte cultures. All the acrocentric chromosome pairs were analyzed using G banding followed by Nucleolus Staining Giemsa banding in the same cell. The Alzheimer's disease patients and elderly healthy control did not show a variation in the transcriptional activity of ribosomal genes with or without 5-azacytidine treatment, whereas the young control group showed an increase in this activity with the treatment.
ISSN:1389-5729
1573-6768
DOI:10.1023/A:1026542618182