Tropomyosin isoforms present in the sea anemone, Anthopleura japonica (Anthozoa, Cnidaria)

Five isoforms of tropomyosin, designated as TMa, TMb, TMc, TMd, and TMe, were detected in the sea anemone, Anthopleura japonica. The apparent molecular weights of these isoforms were estimated to be approximately 30kD to 37.5kD, and their pI values were approximately 4.55 (TMa and TMb) and 4.65 (TMc...

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Bibliographic Details
Published in:The Journal of experimental zoology 2002-12, Vol.293 (7), p.649-663
Main Authors: Fujinoki, Masakatsu, Tomiyama, Toshiko, Ishimoda-Takagi, Tadashi
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Anthopleura japonica
Anthozoa
Anthozoa - anatomy & histology
Anthozoa - chemistry
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Hydrogen-Ion Concentration
Immunoblotting
Immunohistochemistry
Marine
Organ Specificity
Protein Binding
Protein Isoforms - analysis
Protein Isoforms - immunology
Protein Isoforms - metabolism
Rabbits
Tropomyosin - analysis
Tropomyosin - chemistry
Tropomyosin - immunology
Tropomyosin - metabolism
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Summary:Five isoforms of tropomyosin, designated as TMa, TMb, TMc, TMd, and TMe, were detected in the sea anemone, Anthopleura japonica. The apparent molecular weights of these isoforms were estimated to be approximately 30kD to 37.5kD, and their pI values were approximately 4.55 (TMa and TMb) and 4.65 (TMc, TMd, and TMe). Although sea anemone tropomyosin isoforms have the ability to bind to rabbit skeletal muscle actin, they preferably bind to actin at higher concentrations of Mg2+ (10–20 mM) and slightly lower pH (6.2–7.2) than those used in conventional conditions. Antigenic properties of sea anemone tropomyosin seemed to be considerably specific to each isoform. Distribution of tropomyosin isoforms in the sea anemone body was somewhat portion‐specific. TMa, TMb, and TMe were detected similarly in the extracts from tentacle, oral disc, column, mouth, and pedal disc. Although TMc and TMd were detected abundantly in the tentacle extract and moderately in the column and mouth extracts, these components were not contained in the pedal disc extract and detected only faintly in the oral disc extract. J. Exp. Zool. 293:649–663, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:0022-104X
1097-010X
DOI:10.1002/jez.10180