Characterization and Expression of Mammalian Cyclin B3, a Prepachytene Meiotic Cyclin

We report the identification and expression pattern of a full-length human cDNA and a partial mouse cDNA encoding cyclin B3. Cyclin B3 ( CCNB3 ) is conserved from Caenorhabditis elegans to Homo sapiens and has an undefined meiotic function in female, but not male Drosophila melanogaster . We show th...

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Bibliographic Details
Published in:The Journal of biological chemistry 2002-11, Vol.277 (44), p.41960-41969
Main Authors: Nguyen, T B, Manova, K, Capodieci, P, Lindon, C, Bottega, S, Wang, X, Refik-Rogers, J, Pines, J, Wolgemuth, D J, Koff, A
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Nucleus - metabolism
Cyclin B - chemistry
Cyclin B - genetics
Cyclin B - metabolism
Cyclin B1
Cyclins - chemistry
Cyclins - genetics
Drosophila Proteins
Female
Fetus - metabolism
G2 Phase
HeLa Cells
Humans
Male
Meiosis
Mice
Mitosis
Molecular Sequence Data
Ovary - metabolism
RNA, Messenger - analysis
Space life sciences
Spermatogenesis
Testis - metabolism
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Summary:We report the identification and expression pattern of a full-length human cDNA and a partial mouse cDNA encoding cyclin B3. Cyclin B3 ( CCNB3 ) is conserved from Caenorhabditis elegans to Homo sapiens and has an undefined meiotic function in female, but not male Drosophila melanogaster . We show that H. sapiens cyclin B3 interacts with cdk2, is localized to the nucleus, and is degraded during anaphase entry after the degradation of cyclin B1. Degradation is dependent on sequences conserved in a destruction box motif. Overexpression of nondegradable cyclin B3 blocks the mitotic cell cycle in late anaphase, and at higher doses it can interfere with progression through G 1 and entry into S phase. H. sapiens cyclin B3 mRNA and protein are detected readily in developing germ cells in the human testis and not in any other tissue. The mouse cDNA has allowed us to further localize cyclin B3 mRNA to leptotene and zygotene spermatocytes. The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M203951200