Particulate guanylate cyclase and adenylate cyclase activities after activation with various agents in rabbit platelets. An ultracytochemical study

The cytochemical localization of particulate guanylate cyclase and adenylate cyclase activities in rabbit platelets were studied after stimulation with various agents, at the electron microscope level. In the presence of platelet aggregating agents such as thrombin and ADP, the particulate reaction...

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Bibliographic Details
Published in:The Histochemical journal 1991-03, Vol.23 (3), p.143-148
Main Authors: SPRECA, A, RAMBOTTI, M. G, DONATO, R
Format: Article
Language:English
Subjects:
Adenosine Diphosphate - pharmacology
Adenylyl Cyclases - metabolism
Alprostadil - pharmacology
Animals
Atrial Natriuretic Factor - pharmacology
Biological and medical sciences
Blood Platelets - enzymology
Blood Platelets - ultrastructure
Cell Membrane - metabolism
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Guanylate Cyclase - metabolism
Histocytochemistry
Insulin - pharmacology
Microscopy, Electron
Platelet Activation - drug effects
Platelet Activation - physiology
Rabbits
Thrombin - pharmacology
Vertebrates: blood, hematopoietic organs, reticuloendothelial system
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Summary:The cytochemical localization of particulate guanylate cyclase and adenylate cyclase activities in rabbit platelets were studied after stimulation with various agents, at the electron microscope level. In the presence of platelet aggregating agents such as thrombin and ADP, the particulate reaction product of guanylate cyclase activity was detectable on plasma membrane and on membranes of the open canalicular system. In contrast, samples incubated with platelet-activating factor showed no activation of the cyclase activity. Atrial natriuretic factor stimulated the particulate guanylate cyclase. The ultracytochemical localization of this activated cyclase was the same as that of thrombin- or ADP-stimulated guanylate cyclase. Adenylate cyclase activity was studied in platelets incubated with prostaglandin E1 plus or minus insulin. The enzyme reaction product was found at the same sites where guanylate cyclase was detected. Therefore guanylate and adenylate cyclase activities do not seem to be preferentially localised in platelet membranes.
ISSN:0018-2214
1573-6865
DOI:10.1007/BF01047459