AC133+ umbilical cord blood progenitors demonstrate rapid self‐renewal and low apoptosis

Umbilical cord blood (UCB) provides immediate access to haemopoietic stem/progenitor cells (HSPC) but low cell number restricts use in full adult bone marrow reconstitution. This study investigated early ex vivo expansion kinetics of UCB AC133+ cells (2–4 × 104/ml), mononuclear cells (MNC, 1–2 × 106...

Full description

Saved in:
Bibliographic Details
Published in:British journal of haematology 2002-11, Vol.119 (2), p.516-524
Main Authors: Forraz, Nicolas, Pettengell, Ruth, Deglesne, Pierre‐Antoine, McGuckin, Colin P.
Format: Article
Language:English
Subjects:
AC133
AC133 Antigen
ADP-ribosyl Cyclase - immunology
ADP-ribosyl Cyclase 1
Antigens, CD - immunology
Antigens, CD34 - immunology
Apoptosis
Biological and medical sciences
Cell Division
Cell Separation
Cells, Cultured
cord blood
cytokines
Fetal Blood
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glycoproteins - immunology
Hematopoietic Stem Cell Mobilization
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - immunology
Humans
Immunobiology
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Membrane Glycoproteins
Membrane Proteins - pharmacology
Peptides - immunology
stem cells
Thrombopoietin - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Umbilical cord blood (UCB) provides immediate access to haemopoietic stem/progenitor cells (HSPC) but low cell number restricts use in full adult bone marrow reconstitution. This study investigated early ex vivo expansion kinetics of UCB AC133+ cells (2–4 × 104/ml), mononuclear cells (MNC, 1–2 × 106/ml) and AC133negative cells (AC133neg, 2–4 × 104/ml) in stroma‐free 8 d liquid culture (fetal bovine serum‐supplemented Iscove's‐modified Dulbecco's medium (IMDM) with either ‘K36EG’[c‐Kit ligand, interleukin 3 (IL‐3), IL‐6, erythropoietin, granulocyte colony‐stimulating factor] or ‘TPOFL’ (thrombopoietin, Flt‐3 ligand). Cell enumeration, apoptosis assay and AC133/CD34/CD38 antigen immunophenotyping were performed at d 0, 3, 5 and 8. All three cell populations went through a proliferation lag phase between d 3 and d 5. AC133+ cells recovered better from lag phase with significantly higher fold increase (FI) when compared with MNC and AC133neg populations (K36EG FI: 15·04 ± 5·46; TPOFL FI: 8·59 ± 2·92, P 
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.2002.03828.x