Cloning and Expression of cDNAs from Enterically-Transmitted Non-A, Non-B Hepatitis Virus

The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with t...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1991, Vol.35(7), pp.535-543
Main Authors: Ichikawa, Munetaka, Araki, Masatake, Rikihisa, Tetsuji, Uchida, Toshikazu, Shikata, Toshio, Mizuno, Kyosuke
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
Blotting, Southern
Blotting, Western
Cloning, Molecular
Cynomolgus
DNA - genetics
Escherichia coli - genetics
Expression vectors
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetics
Hepatitis Antibodies - analysis
Hepatitis E - immunology
Hepatitis E virus - genetics
Hepatitis E virus - immunology
Humans
Macaca fascicularis
Microbiology
Microscopy, Electron
Molecular Sequence Data
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Viral Proteins - genetics
Viral Proteins - immunology
Virology
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Summary:The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1991.tb01584.x