Self-assembled monolayers of globotriaosylceramide (Gb3) mimics: surface-specific affinity with shiga toxins

Self-assembled monolayers (SAMs) of Gb3 mimics having different lengths of alkyl chains were prepared on gold surfaces, and their interactions with galactose-specific lectin (RCA 120) and Shiga toxins (Stxs) were investigated by a quartz crystal microbalance (QCM) in aqueous solutions. Their interac...

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Bibliographic Details
Published in:Analytical biochemistry 2002-11, Vol.310 (1), p.27-35
Main Authors: Miura, Yoshiko, Sasao, Yuuki, Dohi, Hirofumi, Nishida, Yoshihiro, Kobayashi, Kazukiyo
Format: Article
Language:English
Subjects:
Adsorption
Biomimetic Materials - chemistry
Biomimetic Materials - metabolism
Carbohydrate Conformation
Carbohydrate Sequence
Computer Simulation
Globotriaosylceramide
Gold - chemistry
Kinetics
Models, Molecular
Molecular recognition
Molecular Sequence Data
Oligosaccharides - chemistry
Protein Binding
Quartz crystal microbalance
Self-assembled monolayers
Shiga toxin
Shiga Toxin 1 - metabolism
Shiga Toxin 2 - metabolism
Spectroscopy, Fourier Transform Infrared - methods
Substrate Specificity
Surface Properties
Trihexosylceramides - chemistry
Trihexosylceramides - metabolism
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Summary:Self-assembled monolayers (SAMs) of Gb3 mimics having different lengths of alkyl chains were prepared on gold surfaces, and their interactions with galactose-specific lectin (RCA 120) and Shiga toxins (Stxs) were investigated by a quartz crystal microbalance (QCM) in aqueous solutions. Their interaction with RCA 120 was enhanced owing to the “cluster effect,” regardless of the alkyl chain length of the SAMs. The interaction with Stxs was dependent on the alkyl chain length of Gb3 mimics. Stx-1 and Stx-2 showed a stronger affinity to the Gb3C2 SAM with ethyl disulfide and to the Gb3C10 with decyl disulfide, respectively. Gb3 glycoconjugate polymer with no alkyl spacer inhibited the adsorption of Stx-1 to Gb3C10 SAM but did not inhibit the adsorption of Stx-2 to Gb3C10 SAM. The results suggest that the alkyl chain of the glycolipid takes part in the binding to Stx-2 but not to Stx-1, which is also supported by the computer simulation of Stx-1 with a Gb3 model substance.
ISSN:0003-2697
1096-0309
DOI:10.1016/S0003-2697(02)00318-4