The effect of quercetin on renal ischemia and reperfusion injury in the rat

• Published in: Cell biochemistry and function 2002-12, Vol.20 (4), p.291-296
• Main Authors: Inal, Mine, Altinişik, Mehmet, Bilgin, Mehmet D.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Catalase - metabolism; Erythrocytes - metabolism; Glutathione - metabolism; Glutathione Peroxidase - metabolism; ischemia and reperfusion; Kidney Cortex - metabolism; Male; Malondialdehyde - metabolism; Models, Chemical; Oxidative Stress; quercetin; Quercetin - pharmacology; rat kidney; Rats; Rats, Sprague-Dawley; reactive oxygen species; Reperfusion Injury - drug therapy; Superoxide Dismutase - metabolism
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.953

Renal ischemia–reperfusion injury occurs in many clinical conditions such as hypovolemic shock, thromboembolism, injury and after renal transplantation. Under these conditions, ROS are considered to be the reason for cellular damage. Bioflavonoids have antioxidant and renoprotective properties. We studied the effect of quercetin, a bioflavonoid, on ischemia and reperfusion in rats. The rats (n = 28) were separated into three groups. Group I was the control group. Animals in groups II (IR) and III (IR + Q) underwent 30 min ischemia and 45 min reperfusion, respectively. Rats, in group III, also received 50 mg kg−1 quercetin before 45 min of reperfusion. The activities of SOD, CAT, GPx, and concentrations of GSH and GSSGR were determined in renal cortex and erythrocytes. Also, the levels of MDA in renal cortex and plasma, and XO in renal cortex were measured in these groups. The renal cortex XO levels in the IR group were higher than that of the control and IR+Q groups (p