Zn metabolism affects apoptosis rate and proliferative responsiveness of PBMC from patients on chronic hemodialysis

Patients with end-stage renal failure suffer from severe plasma trace metal deficiency that is not corrected by dialysis. Trace metals, including Zn(2+), are critical for cell differentiation and replication. Zn(2+)also plays important role in cell apoptosis. Both processes are known to be impaired...

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Published in:Metabolism, clinical and experimental clinical and experimental, 2002-11, Vol.51 (11), p.1392-1396
Main Authors: Weissgarten, J, Berman, S, Modai, D, Rosenberg, R, Rapoport, M, Cohen, M, Averbukh, Z
Format: Article
Language:English
Subjects:
Apoptosis
Case-Control Studies
Cell Division
Cells, Cultured
Humans
Kidney Failure, Chronic - blood
Kidney Failure, Chronic - therapy
Monocytes - metabolism
Renal Dialysis
Uremia - blood
Zinc - administration & dosage
Zinc - deficiency
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Summary:Patients with end-stage renal failure suffer from severe plasma trace metal deficiency that is not corrected by dialysis. Trace metals, including Zn(2+), are critical for cell differentiation and replication. Zn(2+)also plays important role in cell apoptosis. Both processes are known to be impaired in uremia. The present study was undertaken to evaluate the effect of Zn(2+) supplementation on apoptosis of cultured peripheral blood mononuclear cells (PBMC) from patients on chronic hemodialysis versus those from healthy control subjects, concomitantly with assessment of mitogen-induced cell proliferation. The results showed that (1) basal total cell-associated Zn(2+) was elevated in uremic PBMC, compared to normal controls (23.9 +/- 5.64 v 10.5 +/- 2.64 micromol/L/mg protein). The gap persisted following incubation in Zn(2+)-enriched medium (63.3 +/- 26.12 v 81.6 +/- 13.4 micromol/L/mg protein, P
ISSN:0026-0495
DOI:10.1053/meta.2002.35575