Pyrococcus furiosus ferredoxin is a functional dimer

Pyrococcus furiosus ferredoxin is subject to a monomer/dimer equilibrium as a function of ionic strength. At physiological ionic strength, approximately 0.35 M NaCl, the protein is very predominantly homodimer. The monomeric form exhibits impaired electron transfer on glassy carbon; it also has a de...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2002-11, Vol.531 (2), p.335-338
Main Authors: Hasan, M.N, Hagedoorn, P.L, Hagen, W.R
Format: Article
Language:English
Subjects:
Chromatography, Gel
Dimer
Dimerization
Electron Spin Resonance Spectroscopy
Electron transfer
Electron Transport
Electrophoresis, Polyacrylamide Gel
Ferredoxin
Ferredoxins - chemistry
Ferredoxins - isolation & purification
Ferredoxins - physiology
Osmolar Concentration
Pyrococcus
Pyrococcus furiosus
Thermophile
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pyrococcus furiosus ferredoxin is subject to a monomer/dimer equilibrium as a function of ionic strength. At physiological ionic strength, approximately 0.35 M NaCl, the protein is very predominantly homodimer. The monomeric form exhibits impaired electron transfer on glassy carbon; it also has a decreased S=3/2 over S=1/2 ratio as shown by electron paramagnetic resonance spectroscopy. Even following sterilization at 121°C the dimer is stable in denaturing gel electrophoresis.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)03554-8