Stereospecific high-performance liquid chromatographic assay of sotalol in plasma

A convenient high-performance liquid chromatographic (HPLC) assay was developed for determination of sotalol (STL) enantiomers in plasma. Following addition of the internal standard (IS; racemic atenolol), enantiomers of STL and IS were extracted using ethyl acetate. After evaporation of the organic...

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Bibliographic Details
Published in:Pharmaceutical research 1991-09, Vol.8 (9), p.1195-1198
Main Authors: CARR, R. A, FOSTER, R. T, BHANJI, N. H
Format: Article
Language:English
Subjects:
Adult
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Cyanates - blood
General pharmacology
Humans
Isocyanates
Male
Medical sciences
Naphthalenes - blood
Pharmacology. Drug treatments
Sotalol - blood
Stereoisomerism
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Summary:A convenient high-performance liquid chromatographic (HPLC) assay was developed for determination of sotalol (STL) enantiomers in plasma. Following addition of the internal standard (IS; racemic atenolol), enantiomers of STL and IS were extracted using ethyl acetate. After evaporation of the organic layer, samples were derivatized with a solution of S-(+)-1-(1-naphthyl)ethyl isocyanate (NEIC). The resulting diastereomers were chromatographed with normal-phase HPLC with chloroform:hexane:methanol [65:33:2 (v/v)] as the mobile phase at a flow rate of 2 ml/min. The fluorescence detection wavelength was set at 220 nm for excitation with no emission filter. The suitability of the assay for pharmacokinetic studies was determined by measuring STL enantiomers in the plasma of a healthy subject after administration of a single 160-mg oral, racemic dose of STL.
ISSN:0724-8741
1573-904X
DOI:10.1023/A:1015870805757