Detection of different developmental stages of malaria parasites by non-radioactive DNA in situ hybridization

A highly sensitive non-radioactive DNA in situ hybridization procedure is described that enables detection and unequivocal identification of various developmental stages of human and rodent malaria parasites. Using biotinylated species-specific DNA probes, erythrocytic parasites can be specifically...

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Bibliographic Details
Published in:The Histochemical journal 1991-03, Vol.23 (3), p.109-115
Main Authors: VAN DEN BERG, F. M, VAN AMSTEL, P. J, JANSE, C. J, MEIS, J. F. G. M, MONS, B
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotin
DNA Probes
DNA, Protozoan - analysis
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Humans
Invertebrates
Liver - parasitology
Malaria - parasitology
Mice
Nucleic Acid Hybridization
Pan troglodytes - parasitology
Plasmodium berghei - genetics
Plasmodium berghei - growth & development
Plasmodium berghei - isolation & purification
Plasmodium falciparum - genetics
Plasmodium falciparum - isolation & purification
Protozoa
Rats
Staining and Labeling - methods
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Summary:A highly sensitive non-radioactive DNA in situ hybridization procedure is described that enables detection and unequivocal identification of various developmental stages of human and rodent malaria parasites. Using biotinylated species-specific DNA probes, erythrocytic parasites can be specifically stained in blood smears. Similarly exoerythrocytic stages can be visualized in cell culture and in sections of paraffin-embedded liver. In blood smears, the hybridization procedure provides a rapid detection of (low) parasitemia and species-determination for experienced microscopists at 100 to 400x magnification. Moreover, the procedure can be applied even after previous Giemsa staining of the preparation, enabling revision of patient smears which were difficult to read after routine Giemsa staining.
ISSN:0018-2214
1573-6865
DOI:10.1007/BF01047455