Different effectors of dimorphism in Yarrowia lipolytica

Yarrowia lipolytica is an ascomycete with biotechnological potential. In common media, the fungus grows as a mixture of yeast-like and short mycelial cells. The environmental factors that affect dimorphism in the wild-type strain, W29, and its auxotrophic derivative, PO1a, were analyzed. In both str...

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Bibliographic Details
Published in:Archives of microbiology 2002-12, Vol.178 (6), p.477-483
Main Authors: Ruiz-Herrera, J, Sentandreu, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
carbon
Carbon - metabolism
citrates
Citric Acid - metabolism
Culture Media
cyclic AMP
Cyclic AMP - pharmacology
dimorphism
environmental factors
Fundamental and applied biological sciences. Psychology
fungi
glucose
Hot Temperature
Hydrogen-Ion Concentration
Microbiology
Morphogenesis
Morphology, structure, chemical composition
mycelium
Mycology
niches
nitrogen
Nitrogen - chemistry
Nitrogen - metabolism
Sex Characteristics
Starvation
Yarrowia - cytology
Yarrowia - drug effects
Yarrowia - growth & development
Yarrowia - physiology
Yarrowia lipolytica
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Summary:Yarrowia lipolytica is an ascomycete with biotechnological potential. In common media, the fungus grows as a mixture of yeast-like and short mycelial cells. The environmental factors that affect dimorphism in the wild-type strain, W29, and its auxotrophic derivative, PO1a, were analyzed. In both strains, pH was the most important factor regulating the dimorphic transition. Mycelium formation was maximal at pH near neutrality and decreased as pH was lowered to become almost null at pH 3. Carbon and nitrogen sources, namely glucose and ammonium, were also important for mycelium formation; and their effect was antagonized by some alternative carbon and nitrogen sources. Citrate was an important positive effector of mycelium growth. Anaerobic stress induced formation of mycelial cells. The importance of the protein kinase A pathway was suggested by the inhibition of mycelium growth by cAMP. We propose that the interplay of these factors regulates the adaptation of the fungus, to better exploit its natural ecological niches.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-002-0478-3