Predictive value of circulating immature cell counts in peripheral blood for timing of peripheral blood progenitor cell collection after G-CSF plus chemotherapy-induced mobilization

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2002-11, Vol.42 (11), p.1514-1522
• Main Authors: Kozuka, Teruhiko, Ikeda, Kazuma, Teshima, Takanori, Kojima, Kensuke, Matsuo, Keitaro, Bessho, Akihiro, Sunami, Kazutaka, Hiramatsu, Yasushi, Maeda, Yoshinobu, Noguchi, Toshio, Yamamoto, Kazuhiko, Fujii, Nobuharu, Imai, Toshi, Takenaka, Katsuto, Shinagawa, Katsuji, Ishimaru, Fumihiko, Niiya, Kenji, Koide, Norio, Tanimoto, Mitsune, Harada, Mine
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Antigens, CD34 - analysis; Antineoplastic Combined Chemotherapy Protocols - administration & dosage; Antineoplastic Combined Chemotherapy Protocols - pharmacology; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; Blood Cell Count; Cellular Senescence; Combined Modality Therapy; Cytarabine - administration & dosage; Cytarabine - pharmacology; Dexamethasone - administration & dosage; Dexamethasone - pharmacology; Etoposide - administration & dosage; Etoposide - pharmacology; Female; Granulocyte Colony-Stimulating Factor - pharmacology; Hematopoietic Stem Cell Mobilization; Hematopoietic Stem Cells - cytology; Humans; Ifosfamide - administration & dosage; Ifosfamide - pharmacology; Leukapheresis; Male; Middle Aged; Neoplasms - blood; Neoplasms - drug therapy; Neoplasms - therapy; Peripheral Blood Stem Cell Transplantation; Predictive Value of Tests; ROC Curve; Transplantation, Autologous
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1046/j.1537-2995.2002.00218.x

BACKGROUND: Enumeration of CD34+ cells in peripheral blood (PB) before apheresis predicts the number of CD34+ cells collected, although flow cytometric techniques used are complex and expensive. In an attempt to determine the optimal timing for peripheral blood progenitor cell (PBPC) collection, the usefulness of circulating immature cell (CIC) counts in PB was evaluated. STUDY DESIGN AND METHODS: CIC counts in PB and CD34+ cell counts in the apheresis product from 249 collections were assessed, and the relationship between these two parameters was evaluated by with the Pearson rank correlation analysis, the Fisher exact test, and the U‐test. RESULTS: CIC counts were correlated significantly with the number of CD34+ cells per kg of patient's body weight in the apheresis product (Pearson rank correlation analysis: r = 0.635, p < 0.0001). When a level of 1 × 109 CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 1 × 106 CD34+ cells per kg of body weight were 75.7 and 85.5 percent, respectively. CONCLUSION: The present study strongly suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1 × 109 per L can be used as a good predictor for PBPC collections containing more than 1 × 106 CD34+ cells per kg of body weight in a single apheresis procedure.