Microdissection and cloning of DNA from landmark loops of amphibian lampbrush chromosomes

Microdissection of the "globular" and "granular" landmark loops of Pleurodeles lampbrush chromosomes and subsequent cloning of their DNA yielded several recombinant clones. The 6.6-kb insert of one of them was subcloned and the 600 bp of one subclone was characterized by Southern...

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Bibliographic Details
Published in:Chromosoma 1991-12, Vol.101 (3), p.180-188
Main Authors: PENRAD-MOBAYED, M, SOURROUILLE, P, BONNANFANT-JAÏS, M. L, N'DA, E, EDSTRÖM, J. E, ANGELIER, N
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Blotting, Southern
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Cloning, Molecular
DNA
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Molecular Sequence Data
Nucleic Acid Hybridization
Oocytes
Pleurodeles - genetics
Restriction Mapping
Transcription, Genetic
Vertebrata
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Summary:Microdissection of the "globular" and "granular" landmark loops of Pleurodeles lampbrush chromosomes and subsequent cloning of their DNA yielded several recombinant clones. The 6.6-kb insert of one of them was subcloned and the 600 bp of one subclone was characterized by Southern and slot hybridizations as well as by sequencing. This sequence, designated p130B, was shown to belong to a class of moderately repetitive DNA. RNA expression of this sequence was investigated by in situ hybridization of p130B to the nascent transcripts of lateral loops. Results showed that: (1) the same transcripts were not always found in matrices of landmarks exhibiting the same morphological features; (2) the same transcripts were expressed in loops of different morphological types. Based on these results we suggest that even if there is a morphological similarity of landmark loops, this does not reflect total similarity of their transcripts.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00355367