Antikeratin 14 monoclonal antibody staining in psoriasis and seborrhoeic keratosis : immunofluorescence and two colour FACS studies

A monoclonal antibody (ES3A) was raised against a mouse graft-versus-host reaction (GVHR) model. This antibody was against basal cell cytoplasm and reacted with an acidic (pI 6.2) 50 kDa keratin of human epidermis. However, ES3A reacted with several lower layers of epidermal cells in psoriasis and s...

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Bibliographic Details
Published in:Archives of Dermatological Research 1991-09, Vol.283 (6), p.405-410
Main Authors: WONGWAISAYAWAN, H, YOSHIIKE, T, AIKAWA, Y, BRIGGAMAN, R. A, OGAWA, H
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Biological and medical sciences
Cell Fusion
Cell Separation
Dermatitis, Seborrheic - immunology
Dermatology
Disease Models, Animal
Epidermal Cells
Flow Cytometry
Fluorescent Antibody Technique
Graft vs Host Reaction - immunology
Humans
Immunoblotting
In Vitro Techniques
Keratins - analysis
Keratins - immunology
Keratosis - immunology
Medical sciences
Mice
Propidium
Psoriasis - immunology
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Summary:A monoclonal antibody (ES3A) was raised against a mouse graft-versus-host reaction (GVHR) model. This antibody was against basal cell cytoplasm and reacted with an acidic (pI 6.2) 50 kDa keratin of human epidermis. However, ES3A reacted with several lower layers of epidermal cells in psoriasis and seborrhoeic keratosis. Acanthotic seborrhoeic keratosis showed varying patterns even in a single lesion. If combined with FACS analysis, ES3A-positive cells could be quantified. Normal skin showed 28%, while psoriasis and seborrhoeic keratosis showed 44% and 51%, respectively. ES3A-positive compartments of the acanthotic type of seborrhoeic keratosis were larger than those of the hyperkeratotic type. ES3A may be suitable for quantification of germinative or proliferative cells.
ISSN:0340-3696
1432-069X
DOI:10.1007/BF00371824