Acute inhibition of glutathione biosynthesis alters endothelial function and blood pressure in rats

• Published in: European journal of pharmacology 2002-11, Vol.454 (2), p.217-223
• Main Authors: Ganafa, Agaba A, Socci, Robin R, Eatman, Danita, Silvestrova, Natalia, Abukhalaf, Imad K, Bayorh, Mohamed A
• Format: Article
• Language: English
• Subjects: Animals; Blood pressure; Blood Pressure - drug effects; Blood Pressure - physiology; Buthionine Sulfoximine - pharmacology; Dose-Response Relationship, Drug; Endothelial function; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Glutathione; Glutathione - antagonists & inhibitors; Glutathione - biosynthesis; Male; Nitric Oxide - metabolism; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - physiology; Rats; Rats, Sprague-Dawley; Space life sciences
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/S0014-2999(02)02500-1

The cardiovascular and biochemical responses during acute oxidative stress induced by d, l-buthionine-( S, R)-sulfoximine (BSO) were investigated in Sprague–Dawley rats. Mean arterial pressure, heart rate and vascular reactivity were measured after subcutaneous injection of BSO (4 mmol/kg). Control rats received saline. Levels of GSH and GSSG in blood and tissues as well as renal superoxide were determined. Nitric oxide, prostacyclin and thromboxane A 2 in plasma and aorta, and isoprostane in plasma were also measured. Blood pressure was elevated at 24 h (121±2 vs. 104±2 mm Hg), with increased reactivity to phenylephrine (by a 59±4 vs. 45±2 mm Hg change), and impaired response to sodium nitroprusside (by a −35±2 vs. −63±2 mm Hg change), P