INHIBITION OF IN VITRO ATTACHMENT OF GIARDIA TROPHOZOITES BY MUCIN

An enzyme-linked immunoabsorbent assay was developed to quantify the number of Giardia sp. trophozoites attached to a substratum. Trophozoites were allowed to attach for 5 or 10 min to untreated or modified substratum, or allowed to attach in the presence of cytoskeletal inhibitors. Microtubule inhi...

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Bibliographic Details
Published in:The Journal of parasitology 2002-10, Vol.88 (5), p.869-873
Main Authors: Roskens, Heidi, Erlandsen, Stanley L
Format: Article
Language:English
Subjects:
Animals
Antiprotozoal Agents - pharmacology
Biological and medical sciences
Cattle
Cell Adhesion - drug effects
Cell Adhesion - physiology
Cell lines
Colchicine - pharmacology
Cytochalasin B - pharmacology
Cytochalasins
Enzyme linked immunosorbent assay
Epithelial cells
Field emission
FUNCTIONAL MORPHOLOGY
Fundamental and applied biological sciences. Psychology
Gastric Mucins - physiology
Giardia lamblia - metabolism
Giardia lamblia - physiology
Giardiasis - metabolism
Giardiasis - parasitology
Intestinal Mucosa - parasitology
Intestinal Mucosa - secretion
Life cycle. Host-agent relationship. Pathogenesis
Microscopy, Electron, Scanning
Microtubules
Mucins
Nocodazole - pharmacology
Optical density
Parasitology
Protozoa
Swine
Trophozoites
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Description
Summary:An enzyme-linked immunoabsorbent assay was developed to quantify the number of Giardia sp. trophozoites attached to a substratum. Trophozoites were allowed to attach for 5 or 10 min to untreated or modified substratum, or allowed to attach in the presence of cytoskeletal inhibitors. Microtubule inhibitors, cochicine and nocodazole, were ineffective in blocking attachment, although the actin-disrupting agent cytochalasin B produced significant inhibition of attachment. Three different mucins were associated with significant inhibition of Giardia trophozoite attachment, which was reversed by treating the mucin-coated wells with 0.1% poly-l-arginine. Examination of mucin-treated substrata by high-resolution field emission scanning electron microscopy showed the presence of thin linear fibrils, whereas treatment of mucin-coated wells with poly-l-arginine revealed similar fibrils but of thicker dimensions. These data support the concept that mucin may inhibit Giardia sp. trophozoite attachment in vitro by electrostatic repulsion and that this can be eliminated by treatment of mucin-coated surfaces with polycationic agents, such as poly-l-arginine or poly-l-lysine.
ISSN:0022-3395
1937-2345
DOI:10.1645/0022-3395(2002)088[0869:IOIVAO]2.0.CO;2