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Identification and Characterization of a cDNA Encoding a Dolichyl Pyrophosphate Phosphatase Located in the Endoplasmic Reticulum of Mammalian Cells
The CWH8 gene in Saccharomyces cerevisiae has been shown recently (Fernandez, F., Rush, J. S., Toke, D. A., Han, G., Quinn, J. E., Carman, G. M., Choi, J.-Y., Voelker, D. R., Aebi, M., and Waechter, C. J. (2001) J. Biol. Chem. 276, 41455â41464) to encode a dolichyl pyrophosphate (Dol-P-P) phosphat...
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Published in: | The Journal of biological chemistry 2002-11, Vol.277 (47), p.45226-45234 |
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description | The CWH8 gene in Saccharomyces cerevisiae has been shown recently (Fernandez, F., Rush, J. S., Toke, D. A., Han, G., Quinn, J. E., Carman, G. M., Choi, J.-Y., Voelker,
D. R., Aebi, M., and Waechter, C. J. (2001) J. Biol. Chem. 276, 41455â41464) to encode a dolichyl pyrophosphate (Dol-P-P) phosphatase associated with crude microsomal fractions. Mutations
in CWH8 result in the accumulation of Dol-P-P, deficiency in lipid intermediate synthesis, defective protein N- glycosylation, and a reduced growth rate. A cDNA ( DOLPP1 , GenBank TM accession number AB030189 ) from mouse brain encoding a homologue of the yeast CWH8 gene is now shown to complement the defects in growth and protein N- glycosylation, and to correct the accumulation of Dol-P-P in the cwh8 Î yeast mutant. Northern blot analyses demonstrate a wide distribution of the DOLPP1 mRNA in mouse tissues. Overexpression of Dolpp1p in yeast, COS, and Sf9 cells produces substantial increases in Dol-P-P phosphatase
activity but not in dolichyl monophosphate or phosphatidic acid phosphatase activities in microsomal fractions. Subcellular
fractionation and immunofluorescence studies localize the enzyme encoded by DOLPP1 to the endoplasmic reticulum of COS cells. The results of protease sensitivity studies with microsomal vesicles from the
lpp1 Î /dpp1 Î yeast mutant expressing DOLPP1 are consistent with Dolpp1p having a luminally oriented active site. The sequence of the DOLPP1 cDNA predicts a polypeptide with 238 amino acids, and a new polypeptide corresponding to 27 kDa is observed when DOLPP1 is expressed in yeast, COS, and Sf9 cells. This study is the first identification and characterization of a cDNA clone encoding
an essential component of a mammalian lipid pyrophosphate phosphatase that is highly specific for Dol-P-P. The specificity,
subcellular location, and topological orientation of the active site described in the current study strongly support a role
for Dolpp1p in the recycling of Dol-P-P discharged during protein N -glycosylation reactions on the luminal leaflet of the endoplasmic reticulum in mammalian cells. |
doi_str_mv | 10.1074/jbc.M207076200 |
format | article |
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D. R., Aebi, M., and Waechter, C. J. (2001) J. Biol. Chem. 276, 41455â41464) to encode a dolichyl pyrophosphate (Dol-P-P) phosphatase associated with crude microsomal fractions. Mutations
in CWH8 result in the accumulation of Dol-P-P, deficiency in lipid intermediate synthesis, defective protein N- glycosylation, and a reduced growth rate. A cDNA ( DOLPP1 , GenBank TM accession number AB030189 ) from mouse brain encoding a homologue of the yeast CWH8 gene is now shown to complement the defects in growth and protein N- glycosylation, and to correct the accumulation of Dol-P-P in the cwh8 Î yeast mutant. Northern blot analyses demonstrate a wide distribution of the DOLPP1 mRNA in mouse tissues. Overexpression of Dolpp1p in yeast, COS, and Sf9 cells produces substantial increases in Dol-P-P phosphatase
activity but not in dolichyl monophosphate or phosphatidic acid phosphatase activities in microsomal fractions. Subcellular
fractionation and immunofluorescence studies localize the enzyme encoded by DOLPP1 to the endoplasmic reticulum of COS cells. The results of protease sensitivity studies with microsomal vesicles from the
lpp1 Î /dpp1 Î yeast mutant expressing DOLPP1 are consistent with Dolpp1p having a luminally oriented active site. The sequence of the DOLPP1 cDNA predicts a polypeptide with 238 amino acids, and a new polypeptide corresponding to 27 kDa is observed when DOLPP1 is expressed in yeast, COS, and Sf9 cells. This study is the first identification and characterization of a cDNA clone encoding
an essential component of a mammalian lipid pyrophosphate phosphatase that is highly specific for Dol-P-P. The specificity,
subcellular location, and topological orientation of the active site described in the current study strongly support a role
for Dolpp1p in the recycling of Dol-P-P discharged during protein N -glycosylation reactions on the luminal leaflet of the endoplasmic reticulum in mammalian cells.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M207076200</identifier><identifier>PMID: 12198133</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Animals ; Brain - physiology ; Cell Line ; Dolichol Phosphates - metabolism ; Endoplasmic Reticulum - enzymology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Genes, Fungal ; Immunohistochemistry ; Mice ; Microsomes - chemistry ; Microsomes - metabolism ; Molecular Sequence Data ; Phosphatidic Acids - metabolism ; Protein Structure, Secondary ; Pyrophosphatases - genetics ; Pyrophosphatases - metabolism ; Rabbits ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Sequence Alignment</subject><ispartof>The Journal of biological chemistry, 2002-11, Vol.277 (47), p.45226-45234</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-557acda4db6f04dbeb0b7d2d91046e6fbbb78dabe153f5a29c4977addfbcfd533</citedby><cites>FETCH-LOGICAL-c426t-557acda4db6f04dbeb0b7d2d91046e6fbbb78dabe153f5a29c4977addfbcfd533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12198133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rush, Jeffrey S</creatorcontrib><creatorcontrib>Cho, Steve K</creatorcontrib><creatorcontrib>Jiang, Songmin</creatorcontrib><creatorcontrib>Hofmann, Sandra L</creatorcontrib><creatorcontrib>Waechter, Charles J</creatorcontrib><title>Identification and Characterization of a cDNA Encoding a Dolichyl Pyrophosphate Phosphatase Located in the Endoplasmic Reticulum of Mammalian Cells</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The CWH8 gene in Saccharomyces cerevisiae has been shown recently (Fernandez, F., Rush, J. S., Toke, D. A., Han, G., Quinn, J. E., Carman, G. M., Choi, J.-Y., Voelker,
D. R., Aebi, M., and Waechter, C. J. (2001) J. Biol. Chem. 276, 41455â41464) to encode a dolichyl pyrophosphate (Dol-P-P) phosphatase associated with crude microsomal fractions. Mutations
in CWH8 result in the accumulation of Dol-P-P, deficiency in lipid intermediate synthesis, defective protein N- glycosylation, and a reduced growth rate. A cDNA ( DOLPP1 , GenBank TM accession number AB030189 ) from mouse brain encoding a homologue of the yeast CWH8 gene is now shown to complement the defects in growth and protein N- glycosylation, and to correct the accumulation of Dol-P-P in the cwh8 Î yeast mutant. Northern blot analyses demonstrate a wide distribution of the DOLPP1 mRNA in mouse tissues. Overexpression of Dolpp1p in yeast, COS, and Sf9 cells produces substantial increases in Dol-P-P phosphatase
activity but not in dolichyl monophosphate or phosphatidic acid phosphatase activities in microsomal fractions. Subcellular
fractionation and immunofluorescence studies localize the enzyme encoded by DOLPP1 to the endoplasmic reticulum of COS cells. The results of protease sensitivity studies with microsomal vesicles from the
lpp1 Î /dpp1 Î yeast mutant expressing DOLPP1 are consistent with Dolpp1p having a luminally oriented active site. The sequence of the DOLPP1 cDNA predicts a polypeptide with 238 amino acids, and a new polypeptide corresponding to 27 kDa is observed when DOLPP1 is expressed in yeast, COS, and Sf9 cells. This study is the first identification and characterization of a cDNA clone encoding
an essential component of a mammalian lipid pyrophosphate phosphatase that is highly specific for Dol-P-P. The specificity,
subcellular location, and topological orientation of the active site described in the current study strongly support a role
for Dolpp1p in the recycling of Dol-P-P discharged during protein N -glycosylation reactions on the luminal leaflet of the endoplasmic reticulum in mammalian cells.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Brain - physiology</subject><subject>Cell Line</subject><subject>Dolichol Phosphates - metabolism</subject><subject>Endoplasmic Reticulum - enzymology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Genes, Fungal</subject><subject>Immunohistochemistry</subject><subject>Mice</subject><subject>Microsomes - chemistry</subject><subject>Microsomes - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Phosphatidic Acids - metabolism</subject><subject>Protein Structure, Secondary</subject><subject>Pyrophosphatases - genetics</subject><subject>Pyrophosphatases - metabolism</subject><subject>Rabbits</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Alignment</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNpFkU1r3DAQhkVpaTZprz0WHUpv3kryh9bHsEnbwOaD0EJvYiSNYgXbciWbsvkb_cNR2IUMYkYzPPMi9BLyibM1Z7L69qjN-lowyWQjGHtDVpxtyqKs-Z-3ZMWY4EUr6s0JOU3pkeWoWv6enHDB2w0vyxX5f2VxnL3zBmYfRgqjpdsOIpgZo386DIOjQM3FzTm9HE2wfnzI_UXoven2Pb3bxzB1IU0dzEjvjjdISHchq6KlfqRzh3nZhqmHNHhD73H2ZumX4UX8GoYBeg8j3WLfpw_knYM-4cdjPSO_v1_-2v4sdrc_rrbnu8JUopmLupZgLFRWN47ljJppaYVtOasabJzWWm4saOR16WoQralaKcFap42zdVmeka8H3SmGvwumWQ0-mfwCGDEsSUnRyKblbQbXB9DEkFJEp6boB4h7xZl6sUFlG9SrDXnh81F50QPaV_z47xn4cgA6_9D98xGV9sF0OCghparyqYVoymf4hpMh</recordid><startdate>20021122</startdate><enddate>20021122</enddate><creator>Rush, Jeffrey S</creator><creator>Cho, Steve K</creator><creator>Jiang, Songmin</creator><creator>Hofmann, Sandra L</creator><creator>Waechter, Charles J</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021122</creationdate><title>Identification and Characterization of a cDNA Encoding a Dolichyl Pyrophosphate Phosphatase Located in the Endoplasmic Reticulum of Mammalian Cells</title><author>Rush, Jeffrey S ; Cho, Steve K ; Jiang, Songmin ; Hofmann, Sandra L ; Waechter, Charles J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-557acda4db6f04dbeb0b7d2d91046e6fbbb78dabe153f5a29c4977addfbcfd533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Brain - physiology</topic><topic>Cell Line</topic><topic>Dolichol Phosphates - metabolism</topic><topic>Endoplasmic Reticulum - enzymology</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Genes, Fungal</topic><topic>Immunohistochemistry</topic><topic>Mice</topic><topic>Microsomes - chemistry</topic><topic>Microsomes - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Phosphatidic Acids - metabolism</topic><topic>Protein Structure, Secondary</topic><topic>Pyrophosphatases - genetics</topic><topic>Pyrophosphatases - metabolism</topic><topic>Rabbits</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rush, Jeffrey S</creatorcontrib><creatorcontrib>Cho, Steve K</creatorcontrib><creatorcontrib>Jiang, Songmin</creatorcontrib><creatorcontrib>Hofmann, Sandra L</creatorcontrib><creatorcontrib>Waechter, Charles J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rush, Jeffrey S</au><au>Cho, Steve K</au><au>Jiang, Songmin</au><au>Hofmann, Sandra L</au><au>Waechter, Charles J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and Characterization of a cDNA Encoding a Dolichyl Pyrophosphate Phosphatase Located in the Endoplasmic Reticulum of Mammalian Cells</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2002-11-22</date><risdate>2002</risdate><volume>277</volume><issue>47</issue><spage>45226</spage><epage>45234</epage><pages>45226-45234</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The CWH8 gene in Saccharomyces cerevisiae has been shown recently (Fernandez, F., Rush, J. S., Toke, D. A., Han, G., Quinn, J. E., Carman, G. M., Choi, J.-Y., Voelker,
D. R., Aebi, M., and Waechter, C. J. (2001) J. Biol. Chem. 276, 41455â41464) to encode a dolichyl pyrophosphate (Dol-P-P) phosphatase associated with crude microsomal fractions. Mutations
in CWH8 result in the accumulation of Dol-P-P, deficiency in lipid intermediate synthesis, defective protein N- glycosylation, and a reduced growth rate. A cDNA ( DOLPP1 , GenBank TM accession number AB030189 ) from mouse brain encoding a homologue of the yeast CWH8 gene is now shown to complement the defects in growth and protein N- glycosylation, and to correct the accumulation of Dol-P-P in the cwh8 Î yeast mutant. Northern blot analyses demonstrate a wide distribution of the DOLPP1 mRNA in mouse tissues. Overexpression of Dolpp1p in yeast, COS, and Sf9 cells produces substantial increases in Dol-P-P phosphatase
activity but not in dolichyl monophosphate or phosphatidic acid phosphatase activities in microsomal fractions. Subcellular
fractionation and immunofluorescence studies localize the enzyme encoded by DOLPP1 to the endoplasmic reticulum of COS cells. The results of protease sensitivity studies with microsomal vesicles from the
lpp1 Î /dpp1 Î yeast mutant expressing DOLPP1 are consistent with Dolpp1p having a luminally oriented active site. The sequence of the DOLPP1 cDNA predicts a polypeptide with 238 amino acids, and a new polypeptide corresponding to 27 kDa is observed when DOLPP1 is expressed in yeast, COS, and Sf9 cells. This study is the first identification and characterization of a cDNA clone encoding
an essential component of a mammalian lipid pyrophosphate phosphatase that is highly specific for Dol-P-P. The specificity,
subcellular location, and topological orientation of the active site described in the current study strongly support a role
for Dolpp1p in the recycling of Dol-P-P discharged during protein N -glycosylation reactions on the luminal leaflet of the endoplasmic reticulum in mammalian cells.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>12198133</pmid><doi>10.1074/jbc.M207076200</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acid Sequence Animals Brain - physiology Cell Line Dolichol Phosphates - metabolism Endoplasmic Reticulum - enzymology Fungal Proteins - genetics Fungal Proteins - metabolism Genes, Fungal Immunohistochemistry Mice Microsomes - chemistry Microsomes - metabolism Molecular Sequence Data Phosphatidic Acids - metabolism Protein Structure, Secondary Pyrophosphatases - genetics Pyrophosphatases - metabolism Rabbits Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Sequence Alignment |
title | Identification and Characterization of a cDNA Encoding a Dolichyl Pyrophosphate Phosphatase Located in the Endoplasmic Reticulum of Mammalian Cells |
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