Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry

Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410,000 theoretical plates) were obtained from low femtomole levels of peptides...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 1991-01, Vol.63 (2), p.109-114
Main Authors: Moseley, M. A, Deterding, L. J, Tomer, K. B, Jorgenson, J. W
Format: Article
Language:English
Subjects:
Absorption
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemotactic Factors - analysis
Electrophoresis - methods
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Molecular Sequence Data
Neuropeptides - analysis
Oligopeptides - analysis
Proteins
Spectrometry, Mass, Fast Atom Bombardment - methods
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410,000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00002a005