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Expression and Location of the Bradykinin B2 Receptor in Rat Testis
To investigate the possible role of the local tissue kallikrein-kinin system in spermatogenesis, we analyzed gene expression and cellular distribution of the bradykinin subtype-2 receptor (B 2 receptor) in the rat testis. Reverse transcription-polymerase chain reaction revealed B 2 receptor expressi...
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| Published in: | Biology of reproduction 2002-12, Vol.67 (6), p.1832-1839 |
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| Main Authors: | , , , , , , , , |
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| Language: | English |
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| container_end_page | 1839 |
| container_issue | 6 |
| container_start_page | 1832 |
| container_title | Biology of reproduction |
| container_volume | 67 |
| creator | Thomas K. Monsees Sonja Blöcher Frank Heidorn Anett Winkler Wolf-Eberhard Siems Werner Müller-Esterl Jaleh Hayatpour Werner Miska Wolf-Bernhard Schill |
| description | To investigate the possible role of the local tissue kallikrein-kinin system in spermatogenesis, we analyzed gene expression
and cellular distribution of the bradykinin subtype-2 receptor (B 2 receptor) in the rat testis. Reverse transcription-polymerase chain reaction revealed B 2 receptor expression in testis and primary cultures of Sertoli cells and peritubular cells isolated from immature and mature
rats. In situ hybridization of the B 2 -receptor mRNA showed intense labeling of cells on the base of the seminiferous tubule, whereas the autoradiographic signals
gradually decreased toward the lumen. Immune histochemistry using testicular sections of pubertal and adult rats showed specific
staining for the B 2 -receptor protein in cells of the adluminal compartment of the seminiferous tubules, especially on pachytene spermatocytes
and spermatids. This immunostaining varied with the stages of the seminiferous cycle. The receptor protein was also observed
on peritubular cells of pubertal rats. In conclusion, we demonstrated a stage-specific expression of the bradykinin B 2 receptor in different cells of the seminiferous tubules of the rat testis. The results point to a possible function of the
tissue kallikrein-kinin system in the local regulation of spermatogenesis. |
| doi_str_mv | 10.1095/biolreprod.102.005207 |
| format | article |
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and cellular distribution of the bradykinin subtype-2 receptor (B 2 receptor) in the rat testis. Reverse transcription-polymerase chain reaction revealed B 2 receptor expression in testis and primary cultures of Sertoli cells and peritubular cells isolated from immature and mature
rats. In situ hybridization of the B 2 -receptor mRNA showed intense labeling of cells on the base of the seminiferous tubule, whereas the autoradiographic signals
gradually decreased toward the lumen. Immune histochemistry using testicular sections of pubertal and adult rats showed specific
staining for the B 2 -receptor protein in cells of the adluminal compartment of the seminiferous tubules, especially on pachytene spermatocytes
and spermatids. This immunostaining varied with the stages of the seminiferous cycle. The receptor protein was also observed
on peritubular cells of pubertal rats. In conclusion, we demonstrated a stage-specific expression of the bradykinin B 2 receptor in different cells of the seminiferous tubules of the rat testis. The results point to a possible function of the
tissue kallikrein-kinin system in the local regulation of spermatogenesis.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.102.005207</identifier><identifier>PMID: 12444060</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; DNA, Complementary - analysis ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Immunohistochemistry ; In Situ Hybridization ; Male ; Mammalian male genital system ; Morphology. Physiology ; Rats ; Rats, Sprague-Dawley ; Receptor, Bradykinin B2 ; Receptors, Bradykinin - analysis ; Receptors, Bradykinin - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Seminiferous Tubules - chemistry ; Seminiferous Tubules - cytology ; Sertoli Cells - chemistry ; Sexual Maturation ; Spermatids - chemistry ; Spermatocytes - chemistry ; Spermatogenesis ; Testis - chemistry ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2002-12, Vol.67 (6), p.1832-1839</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14369431$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12444060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas K. Monsees</creatorcontrib><creatorcontrib>Sonja Blöcher</creatorcontrib><creatorcontrib>Frank Heidorn</creatorcontrib><creatorcontrib>Anett Winkler</creatorcontrib><creatorcontrib>Wolf-Eberhard Siems</creatorcontrib><creatorcontrib>Werner Müller-Esterl</creatorcontrib><creatorcontrib>Jaleh Hayatpour</creatorcontrib><creatorcontrib>Werner Miska</creatorcontrib><creatorcontrib>Wolf-Bernhard Schill</creatorcontrib><title>Expression and Location of the Bradykinin B2 Receptor in Rat Testis</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>To investigate the possible role of the local tissue kallikrein-kinin system in spermatogenesis, we analyzed gene expression
and cellular distribution of the bradykinin subtype-2 receptor (B 2 receptor) in the rat testis. Reverse transcription-polymerase chain reaction revealed B 2 receptor expression in testis and primary cultures of Sertoli cells and peritubular cells isolated from immature and mature
rats. In situ hybridization of the B 2 -receptor mRNA showed intense labeling of cells on the base of the seminiferous tubule, whereas the autoradiographic signals
gradually decreased toward the lumen. Immune histochemistry using testicular sections of pubertal and adult rats showed specific
staining for the B 2 -receptor protein in cells of the adluminal compartment of the seminiferous tubules, especially on pachytene spermatocytes
and spermatids. This immunostaining varied with the stages of the seminiferous cycle. The receptor protein was also observed
on peritubular cells of pubertal rats. In conclusion, we demonstrated a stage-specific expression of the bradykinin B 2 receptor in different cells of the seminiferous tubules of the rat testis. The results point to a possible function of the
tissue kallikrein-kinin system in the local regulation of spermatogenesis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>DNA, Complementary - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptor, Bradykinin B2</subject><subject>Receptors, Bradykinin - analysis</subject><subject>Receptors, Bradykinin - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Seminiferous Tubules - chemistry</subject><subject>Seminiferous Tubules - cytology</subject><subject>Sertoli Cells - chemistry</subject><subject>Sexual Maturation</subject><subject>Spermatids - chemistry</subject><subject>Spermatocytes - chemistry</subject><subject>Spermatogenesis</subject><subject>Testis - chemistry</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNpFj0tLw0AUhQdRbK3-BCUb3aXOK5NkqaU-oCCU7sPNzI0ZzaPOpMT-e0esdHU48HEOHyHXjM4ZzZP70vaNw63rTeh8TmnCaXpCpizheZxylZ2SKaVUxUIoMSEX3n9QyqTg4pxMGJdSUkWnZLH83jr03vZdBJ2JVr2G4bf0VTTUGD06MPtP29kueuTRGjVuh95Foa5hiDboB-svyVkFjcerQ87I5mm5WbzEq7fn18XDKq5ZJoaYa5kwoUuQVCcmExxMUgpENFVVKQ2lQQShULKMITNZlWmVpqLUkCUctJiRu7_ZIP21C89Fa73GpoEO-50vgnSe5FwG8OYA7soWTbF1tgW3L_6tA3B7AMBraCoHnbb-yEmhcinY8bG27_VoHRa-haYJs6IYx1GlhSqCGhc_fhB2uA</recordid><startdate>200212</startdate><enddate>200212</enddate><creator>Thomas K. 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Physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptor, Bradykinin B2</topic><topic>Receptors, Bradykinin - analysis</topic><topic>Receptors, Bradykinin - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Seminiferous Tubules - chemistry</topic><topic>Seminiferous Tubules - cytology</topic><topic>Sertoli Cells - chemistry</topic><topic>Sexual Maturation</topic><topic>Spermatids - chemistry</topic><topic>Spermatocytes - chemistry</topic><topic>Spermatogenesis</topic><topic>Testis - chemistry</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas K. 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Monsees</au><au>Sonja Blöcher</au><au>Frank Heidorn</au><au>Anett Winkler</au><au>Wolf-Eberhard Siems</au><au>Werner Müller-Esterl</au><au>Jaleh Hayatpour</au><au>Werner Miska</au><au>Wolf-Bernhard Schill</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and Location of the Bradykinin B2 Receptor in Rat Testis</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-12</date><risdate>2002</risdate><volume>67</volume><issue>6</issue><spage>1832</spage><epage>1839</epage><pages>1832-1839</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>To investigate the possible role of the local tissue kallikrein-kinin system in spermatogenesis, we analyzed gene expression
and cellular distribution of the bradykinin subtype-2 receptor (B 2 receptor) in the rat testis. Reverse transcription-polymerase chain reaction revealed B 2 receptor expression in testis and primary cultures of Sertoli cells and peritubular cells isolated from immature and mature
rats. In situ hybridization of the B 2 -receptor mRNA showed intense labeling of cells on the base of the seminiferous tubule, whereas the autoradiographic signals
gradually decreased toward the lumen. Immune histochemistry using testicular sections of pubertal and adult rats showed specific
staining for the B 2 -receptor protein in cells of the adluminal compartment of the seminiferous tubules, especially on pachytene spermatocytes
and spermatids. This immunostaining varied with the stages of the seminiferous cycle. The receptor protein was also observed
on peritubular cells of pubertal rats. In conclusion, we demonstrated a stage-specific expression of the bradykinin B 2 receptor in different cells of the seminiferous tubules of the rat testis. The results point to a possible function of the
tissue kallikrein-kinin system in the local regulation of spermatogenesis.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12444060</pmid><doi>10.1095/biolreprod.102.005207</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2002-12, Vol.67 (6), p.1832-1839 |
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| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Biological and medical sciences Cells, Cultured DNA, Complementary - analysis Fundamental and applied biological sciences. Psychology Gene Expression Immunohistochemistry In Situ Hybridization Male Mammalian male genital system Morphology. Physiology Rats Rats, Sprague-Dawley Receptor, Bradykinin B2 Receptors, Bradykinin - analysis Receptors, Bradykinin - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Seminiferous Tubules - chemistry Seminiferous Tubules - cytology Sertoli Cells - chemistry Sexual Maturation Spermatids - chemistry Spermatocytes - chemistry Spermatogenesis Testis - chemistry Vertebrates: reproduction |
| title | Expression and Location of the Bradykinin B2 Receptor in Rat Testis |
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