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Expression of Pejerrey Gonadotropin-Releasing Hormone in Three Orders of Fish

Molecular variants of GnRH were characterized by reverse-phase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [ Synbranchus marmoratus ]), Cyprinidontiformes (platyfish [ Xiphophorus maculatus ] and green swordtail [ X. hell...

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Published in:Biology of reproduction 2002-12, Vol.67 (6), p.1864-1871
Main Authors: SOMOZA, G. M, LESCHEID, D. W, MIRANDA, L. A, LO NOSTRO, F. L, MAGLIULO-CEPRIANO, L, MONTANER, A. D, SCHREIBMAN, M. P, RIVIER, J. E, SHERWOOD, N. M
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container_issue 6
container_start_page 1864
container_title Biology of reproduction
container_volume 67
creator SOMOZA, G. M
LESCHEID, D. W
MIRANDA, L. A
LO NOSTRO, F. L
MAGLIULO-CEPRIANO, L
MONTANER, A. D
SCHREIBMAN, M. P
RIVIER, J. E
SHERWOOD, N. M
description Molecular variants of GnRH were characterized by reverse-phase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [ Synbranchus marmoratus ]), Cyprinidontiformes (platyfish [ Xiphophorus maculatus ] and green swordtail [ X. helleri ]), and Atheriniformes (Patagonia pejerrey [ Odontesthes hatchery ]). Also, pituitary gland extracts from the pejerrey O. bonariensis (Atheriniformes) were characterized. Eluted fractions were tested in radioimmunoassays with antisera specific to GnRH, including both antisera that detected only one form of GnRH and those that detected several forms. The results show that brain extracts obtained from all species contained the same three molecular forms of GnRH, which were immunologically and chromatographically undistinguishable from chicken GnRH-II, pejerrey GnRH (pjGnRH), and salmon GnRH. This study supports the hypothesis that expression of these three forms is common in different fish orders and that pjGnRH is the main regulator of pituitary function in these fish.
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source Oxford Journals Online
subjects Animals
Biological and medical sciences
Brain Chemistry
Chromatography, High Pressure Liquid
Cyprinodontiformes - metabolism
Female
Fundamental and applied biological sciences. Psychology
Gene Expression
Gonadotropin-Releasing Hormone - genetics
Gonadotropin-Releasing Hormone - isolation & purification
Hypothalamus. Hypophysis. Epiphysis. Urophysis
Male
Morphology. Functional localizations
Pituitary Gland - chemistry
Smegmamorpha - metabolism
Space life sciences
Tissue Extracts - chemistry
Vertebrates: endocrinology
title Expression of Pejerrey Gonadotropin-Releasing Hormone in Three Orders of Fish
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