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Age-related changes of the nitric oxide system in the rat brain

This work examines the age-related changes of the NO pathway in the central nervous system (CNS), analyzing nitric oxide synthase (NOS) isoform expression, the level of nitrotyrosine-modified proteins, and the NOS activity in the cerebral cortex, decorticated brain (basal ganglia, thalamus, hypothal...

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Published in:Brain research 2002-11, Vol.956 (2), p.385-392
Main Authors: Siles, Eva, Martı́nez-Lara, Esther, Cañuelo, Ana, Sánchez, Marta, Hernández, Raquel, López-Ramos, Juan Carlos, Del Moral, Marı́a Luisa, Esteban, Francisco José, Blanco, Santos, Pedrosa, Juan Angel, Rodrigo, José, Peinado, Marı́a Angeles
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cited_by cdi_FETCH-LOGICAL-c488t-cd3f1b340f95e1eea3ba8c12745e74efac9b48360f9c7f9d8cba65878806afa13
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creator Siles, Eva
Martı́nez-Lara, Esther
Cañuelo, Ana
Sánchez, Marta
Hernández, Raquel
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Del Moral, Marı́a Luisa
Esteban, Francisco José
Blanco, Santos
Pedrosa, Juan Angel
Rodrigo, José
Peinado, Marı́a Angeles
description This work examines the age-related changes of the NO pathway in the central nervous system (CNS), analyzing nitric oxide synthase (NOS) isoform expression, the level of nitrotyrosine-modified proteins, and the NOS activity in the cerebral cortex, decorticated brain (basal ganglia, thalamus, hypothalamus, tegtum and tegmentum) and cerebellum of young, adult and aged rats. Our data demonstrate that the different NOS isoforms are not uniformly expressed across the CNS. In this sense, the nNOS and eNOS isoenzymes are expressed mainly in the cerebellum and decorticated brain, respectively, while the iNOS isoenzyme shows the highest level in cerebellum. Concerning age, in the cerebral cortex nNOS significantly increased its expression only in adult animals; meanwhile, in the cerebellum the eNOS expression decreased whereas iNOS increased in adult and aged rats. No age-related changes in any isoform were found in decorticated brain. NOS activity, determined by nitrate plus nitrite quantification, registered the highest levels in the cerebellum, where the significant increase detected with aging was probably related to iNOS activity. The number of nitrotyrosine-modified immunoreactive bands differed among regions; thus, the highest number was detected in the decorticated brain while the cerebellum showed the least number of bands. Finally, bulk protein nitration increased in cerebral cortex only in adult animal. No changes were found in the decorticated brain, and the decrease detected in the cerebellum of aged animals was not significant. According to these results, the NO pathway is differently modified with age in the three CNS regions analyzed.
doi_str_mv 10.1016/S0006-8993(02)03575-8
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ispartof Brain research, 2002-11, Vol.956 (2), p.385-392
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subjects Aging
Aging - metabolism
Animals
Biological and medical sciences
Blotting, Western
Brain - enzymology
Brain - metabolism
Cerebral Decortication
Development. Senescence. Regeneration. Transplantation
eNOS
Fundamental and applied biological sciences. Psychology
iNOS
Male
Nitrate/nitrite quantification
Nitrates - metabolism
Nitric Oxide - metabolism
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Nitrites - metabolism
Nitrotyrosine-modified protein
nNOS
Rats
Rats, Wistar
Vertebrates: nervous system and sense organs
title Age-related changes of the nitric oxide system in the rat brain
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