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Structural analysis of the oligosaccharide alditols released from the jelly coat of Rana dalmatina eggs by reductive β-elimination
A combination of ion-exchange chromatography and high performance liquid chromatography (HPLC) has been used to separate the reduced oligosaccharides produced by alkaline borohydride degradation of oviducal mucins obtained from the jelly coat of Rana dalmatina. The primary structures of 26 O-glycans...
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| Published in: | Biochimie 2002-07, Vol.84 (7), p.611-624 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c392t-9198337da3ddb2ecbdf8ba58da1dc1463b264ff3414c07620196de7e1c43d6323 |
| container_end_page | 624 |
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| container_start_page | 611 |
| container_title | Biochimie |
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| creator | Florea, Doina Maes, Emmanuel Haddad, Mohamed Strecker, Gérard |
| description | A combination of ion-exchange chromatography and high performance liquid chromatography (HPLC) has been used to separate the reduced oligosaccharides produced by alkaline borohydride degradation of oviducal mucins obtained from the jelly coat of
Rana dalmatina. The primary structures of 26
O-glycans were determined by one-dimensional and two-dimensional
1H and
1H
13C NMR spectroscopy. As observed for 20 other amphibian species, these carbohydrate chains are highly species-specific. The main typical feature of the species
R.
dalmatina consists in the presence of the backbone Gal(β1-3)[Gal(β1-4)]Gal(β1-3)GalNAc-ol, previously observed among Ranidae, such as
R.
temporaria and
R.
ridibunda. Nevertheless, the nature of carbohydrates present at the periphery of the glycans perfectly differentiates the three species. |
| doi_str_mv | 10.1016/S0300-9084(02)01432-3 |
| format | article |
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Rana dalmatina. The primary structures of 26
O-glycans were determined by one-dimensional and two-dimensional
1H and
1H
13C NMR spectroscopy. As observed for 20 other amphibian species, these carbohydrate chains are highly species-specific. The main typical feature of the species
R.
dalmatina consists in the presence of the backbone Gal(β1-3)[Gal(β1-4)]Gal(β1-3)GalNAc-ol, previously observed among Ranidae, such as
R.
temporaria and
R.
ridibunda. Nevertheless, the nature of carbohydrates present at the periphery of the glycans perfectly differentiates the three species.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/S0300-9084(02)01432-3</identifier><identifier>PMID: 12453633</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Amphibians ; Animals ; Borohydrides - metabolism ; Carbohydrate Conformation ; Carbohydrate Sequence ; Carbohydrates ; Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; Molecular Sequence Data ; Mucins ; Mucins - chemistry ; NMR ; Nuclear Magnetic Resonance, Biomolecular ; Oligosaccharides - chemistry ; Oligosaccharides - isolation & purification ; Oocytes - metabolism ; Rana dalmatina ; Ranidae ; Ranidae - metabolism ; Species Specificity ; Sugar Alcohols - chemistry ; Sugar Alcohols - isolation & purification</subject><ispartof>Biochimie, 2002-07, Vol.84 (7), p.611-624</ispartof><rights>2002 Société française de biochimie et biologie moléculaire / Éditions scientifiques et médicales Elsevier SAS</rights><rights>Copyright 2002 Société françcaise de biochimie et biologie moléculaire / Editions scientifiques et médicales Elsevier SAS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-9198337da3ddb2ecbdf8ba58da1dc1463b264ff3414c07620196de7e1c43d6323</citedby><cites>FETCH-LOGICAL-c392t-9198337da3ddb2ecbdf8ba58da1dc1463b264ff3414c07620196de7e1c43d6323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12453633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Florea, Doina</creatorcontrib><creatorcontrib>Maes, Emmanuel</creatorcontrib><creatorcontrib>Haddad, Mohamed</creatorcontrib><creatorcontrib>Strecker, Gérard</creatorcontrib><title>Structural analysis of the oligosaccharide alditols released from the jelly coat of Rana dalmatina eggs by reductive β-elimination</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>A combination of ion-exchange chromatography and high performance liquid chromatography (HPLC) has been used to separate the reduced oligosaccharides produced by alkaline borohydride degradation of oviducal mucins obtained from the jelly coat of
Rana dalmatina. The primary structures of 26
O-glycans were determined by one-dimensional and two-dimensional
1H and
1H
13C NMR spectroscopy. As observed for 20 other amphibian species, these carbohydrate chains are highly species-specific. The main typical feature of the species
R.
dalmatina consists in the presence of the backbone Gal(β1-3)[Gal(β1-4)]Gal(β1-3)GalNAc-ol, previously observed among Ranidae, such as
R.
temporaria and
R.
ridibunda. Nevertheless, the nature of carbohydrates present at the periphery of the glycans perfectly differentiates the three species.</description><subject>Amphibians</subject><subject>Animals</subject><subject>Borohydrides - metabolism</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Carbohydrates</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Ion Exchange</subject><subject>Molecular Sequence Data</subject><subject>Mucins</subject><subject>Mucins - chemistry</subject><subject>NMR</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - isolation & purification</subject><subject>Oocytes - metabolism</subject><subject>Rana dalmatina</subject><subject>Ranidae</subject><subject>Ranidae - metabolism</subject><subject>Species Specificity</subject><subject>Sugar Alcohols - chemistry</subject><subject>Sugar Alcohols - isolation & purification</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkc9KHTEUh0NR6q3tI7RkJe1ibJIzk5lZSZFWC4Kg7TpkkjPXSOZGk4xw132jPojPZO4f2qWrBPL9zu-Qj5CPnJ1yxuXXWwaMVT3r6s9MfGG8BlHBG7LgErpK8g4OyOIfckTepXTPGGuY6N-SIy7qBiTAgvy5zXE2eY7aU73Sfp1comGk-Q5p8G4ZkjbmTkdnkWpvXQ4-0YgedUJLxximLXqP3q-pCTpvwjdlErXaTzq7csPlMtFhXWK2VLknpM9_K_RuKo_ZhdV7cjhqn_DD_jwmv398_3V-WV1dX_w8_3ZVGehFrnredwCt1WDtINAMduwG3XRWc2t4LWEQsh5HqHltWCsF47202CI3NVgJAo7JyW7uQwyPM6asJpdM2VyvMMxJtaLlvBHNq6BgIDreQgGbHWhiSCniqB6im3RcK87URpPaalIbB4oJtdWkNrlP-4J5mND-T-29FOBsB2D5jyeHUSXjcGXQuogmKxvcKxUvIiGkng</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Florea, Doina</creator><creator>Maes, Emmanuel</creator><creator>Haddad, Mohamed</creator><creator>Strecker, Gérard</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Structural analysis of the oligosaccharide alditols released from the jelly coat of Rana dalmatina eggs by reductive β-elimination</title><author>Florea, Doina ; Maes, Emmanuel ; Haddad, Mohamed ; Strecker, Gérard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-9198337da3ddb2ecbdf8ba58da1dc1463b264ff3414c07620196de7e1c43d6323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amphibians</topic><topic>Animals</topic><topic>Borohydrides - metabolism</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Carbohydrates</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Ion Exchange</topic><topic>Molecular Sequence Data</topic><topic>Mucins</topic><topic>Mucins - chemistry</topic><topic>NMR</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - isolation & purification</topic><topic>Oocytes - metabolism</topic><topic>Rana dalmatina</topic><topic>Ranidae</topic><topic>Ranidae - metabolism</topic><topic>Species Specificity</topic><topic>Sugar Alcohols - chemistry</topic><topic>Sugar Alcohols - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Florea, Doina</creatorcontrib><creatorcontrib>Maes, Emmanuel</creatorcontrib><creatorcontrib>Haddad, Mohamed</creatorcontrib><creatorcontrib>Strecker, Gérard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Florea, Doina</au><au>Maes, Emmanuel</au><au>Haddad, Mohamed</au><au>Strecker, Gérard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural analysis of the oligosaccharide alditols released from the jelly coat of Rana dalmatina eggs by reductive β-elimination</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>84</volume><issue>7</issue><spage>611</spage><epage>624</epage><pages>611-624</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><abstract>A combination of ion-exchange chromatography and high performance liquid chromatography (HPLC) has been used to separate the reduced oligosaccharides produced by alkaline borohydride degradation of oviducal mucins obtained from the jelly coat of
Rana dalmatina. The primary structures of 26
O-glycans were determined by one-dimensional and two-dimensional
1H and
1H
13C NMR spectroscopy. As observed for 20 other amphibian species, these carbohydrate chains are highly species-specific. The main typical feature of the species
R.
dalmatina consists in the presence of the backbone Gal(β1-3)[Gal(β1-4)]Gal(β1-3)GalNAc-ol, previously observed among Ranidae, such as
R.
temporaria and
R.
ridibunda. Nevertheless, the nature of carbohydrates present at the periphery of the glycans perfectly differentiates the three species.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>12453633</pmid><doi>10.1016/S0300-9084(02)01432-3</doi><tpages>14</tpages></addata></record> |
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| identifier | ISSN: 0300-9084 |
| ispartof | Biochimie, 2002-07, Vol.84 (7), p.611-624 |
| issn | 0300-9084 1638-6183 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72711525 |
| source | ScienceDirect Freedom Collection |
| subjects | Amphibians Animals Borohydrides - metabolism Carbohydrate Conformation Carbohydrate Sequence Carbohydrates Chromatography, High Pressure Liquid Chromatography, Ion Exchange Molecular Sequence Data Mucins Mucins - chemistry NMR Nuclear Magnetic Resonance, Biomolecular Oligosaccharides - chemistry Oligosaccharides - isolation & purification Oocytes - metabolism Rana dalmatina Ranidae Ranidae - metabolism Species Specificity Sugar Alcohols - chemistry Sugar Alcohols - isolation & purification |
| title | Structural analysis of the oligosaccharide alditols released from the jelly coat of Rana dalmatina eggs by reductive β-elimination |
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