Intercellular Communication in Cultured Rabbit Gastric Epithelial Cells

The effects of drugs related to cyclic AMP and a tumor promoter, phorbol ester, on intercellular communications via gap junctions were investigated by the Lucifer Yellow-transfer method in cultured rabbit gastric epithelial cells. Cells were in contact with each drug for 4 hr before the microinjecti...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1991-01, Vol.57 (3), p.321-328
Main Authors: Ueda, Fusao, Kyoi, Takashi, Mimura, Kiyotaka, Kimura, Kiyoshi, Yamamoto, Masao
Format: Article
Language:English
Subjects:
Animals
Anti-Ulcer Agents - pharmacology
Biological and medical sciences
Cell Communication - drug effects
Cell Communication - physiology
Cells, Cultured
communication
Cyclic AMP - analysis
Cyclic AMP - physiology
Epithelial Cells
epithelium
Epithelium - drug effects
Epithelium - metabolism
Extracellular Space - physiology
Fundamental and applied biological sciences. Psychology
gap junctions
Gastric Mucosa - chemistry
Gastric Mucosa - metabolism
Intercellular Junctions - drug effects
Intercellular Junctions - physiology
Isoquinolines - metabolism
Microscopy, Electron
Rabbits
Stomach
Stomach - cytology
Stomach - drug effects
Triazines - pharmacology
Up-Regulation - physiology
Vertebrates: digestive system
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Summary:The effects of drugs related to cyclic AMP and a tumor promoter, phorbol ester, on intercellular communications via gap junctions were investigated by the Lucifer Yellow-transfer method in cultured rabbit gastric epithelial cells. Cells were in contact with each drug for 4 hr before the microinjection of the dye into a cell. Dye transfer capacity was significantly increased by dibutyryl cyclic AMP (10-3 M), theophylline (10-3 M), 3-isobutyl-1-methylxanthine (10-4 M), forskolin (10-6 M) and irsogladine (10-4 M); and it was inhibited by 12-0-tetradecanoyl-phorbol-13-acetate (100 ng/ml). These results suggest that the intercellular communication between cultured rabbit gastric epithelial cells is upregulated by cyclic AMP.
ISSN:0021-5198
1347-3506
DOI:10.1016/S0021-5198(19)39816-6