Variation of variable number of tandem repeat sequences in the 3'-untranslated region of primate dopamine transporter genes that affects reporter gene expression

Genetic polymorphism has been reported in the 3'-untranslated region (3'-UTR) of the human dopamine transporter (DAT) gene and the variable number of tandem repeat (VNTR) polymorphism has been proposed to be associated with normal personal traits or psychoneurological disorders. To assess...

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Bibliographic Details
Published in:Neuroscience letters 2002-12, Vol.334 (3), p.206-210
Main Authors: INOUE-MURAYAMA, Miho, ADACHI, Satomi, MISHIMA, Noriko, MITANI, Hiroaki, TAKENAKA, Osamu, TERAOC, Keiji, HAYASAKA, Ikuo, ITO, Shin'Ichi, MURAYAMA, Yuichi
Format: Article
Language:English
Subjects:
3' Untranslated Regions - genetics
Analysis of Variance
Animals
Biological and medical sciences
Cell Line
Cloning, Molecular
DNA Primers
Dopamine Plasma Membrane Transport Proteins
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes, Reporter
Genes. Genome
Genetic Variation
Gorilla gorilla
Hominidae
Humans
Luciferases - genetics
Membrane Glycoproteins
Membrane Transport Proteins - genetics
Minisatellite Repeats - genetics
Molecular and cellular biology
Molecular genetics
Nerve Tissue Proteins
Neuroblastoma
Polymerase Chain Reaction
Polymorphism, Genetic
Pongo pygmaeus
Primates
Species Specificity
Transfection
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Summary:Genetic polymorphism has been reported in the 3'-untranslated region (3'-UTR) of the human dopamine transporter (DAT) gene and the variable number of tandem repeat (VNTR) polymorphism has been proposed to be associated with normal personal traits or psychoneurological disorders. To assess the variation of this region in nonhuman primates, we amplified the VNTR regions by the polymerase chain reaction in several species of apes and monkeys, and determined their DNA sequences. The 3'-UTR of the chimpanzee DAT gene was also polymorphic and alleles with one or two unit(s) of a 40 bp sequence were found, while all gorillas and orangutans examined had only 2-repeat allele. Cynomolgus macaques and African green monkeys shared 11- or 12-repeat and 5-repeat alleles, respectively. By performing transient transfection assay, we found that most of the VNTR sequences of nonhuman primates exhibited higher activities on reporter gene assay as compared to those of human 9-, 10- and 11-repeat sequences.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(02)01125-4